Studies with a Growth Hormone Antagonist and Dual-fluorescent Confocal Microscopy Demonstrate that the Full-length Human Growth Hormone Receptor, but Not the Truncated Isoform, Is Very Rapidly Internalized Independent of Jak2-Stat5 Signaling

We have investigated trafficking of two negative regulators of growth hormone receptor (GHR) signaling: a human, truncated receptor, GHR1–279, and a GH antagonist, B2036. Fluorescent-labeled growth hormone (GH) was rapidly internalized by the full-length GHR, with >80% of the hormone internalized...

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Bibliographic Details
Published in:The Journal of biological chemistry 1999-05, Vol.274 (21), p.14791-14798
Main Authors: Maamra, Mabrouka, Finidori, Joelle, Von Laue, Sigward, Simon, Sylvie, Justice, Sue, Webster, Jonathan, Dower, Steven, Ross, Richard
Format: Article
Language:English
Subjects:
Golgi Apparatus - physiology
Human Growth Hormone - antagonists & inhibitors
Humans
Microscopy, Confocal
Microscopy, Fluorescence
Protein Isoforms
Receptors, Somatotropin - drug effects
Receptors, Somatotropin - physiology
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Summary:We have investigated trafficking of two negative regulators of growth hormone receptor (GHR) signaling: a human, truncated receptor, GHR1–279, and a GH antagonist, B2036. Fluorescent-labeled growth hormone (GH) was rapidly internalized by the full-length GHR, with >80% of the hormone internalized within 5 min of exposure to GH. In contrast,
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.274.21.14791