Intraspecific ITS polymorphism in Scutellospora castanea (glomales, zygomycota) is structured within multinucleate spores

Polymorphism of the internal transcribed spacers (ITS) of the ribosomal DNA in Scutellospora castanea (Glomales, Zygomycota) and its organization among spores were evaluated. Polymerase chain reaction (PCR) amplification with ITS1/ITS4 primers yielded several fragments of different lengths, even fro...

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Bibliographic Details
Published in:Fungal genetics and biology 1999-03, Vol.26 (2), p.141-151
Main Authors: Hijri, M, Hosny, M, Tuinen, D. van, Dulieu, H
Format: Article
Language:English
Subjects:
Base Sequence
DNA, Fungal - genetics
DNA, Ribosomal - genetics
Endogonales
fungal spores
Fungi - genetics
Fungi - physiology
genbank/aj002871
genbank/aj002872
genbank/aj002873
genbank/aj002874
genbank/aj002875
genetic polymorphism
gnebank/aj002876
internal transcribed spacers
Molecular Sequence Data
mycorrhizal fungi
nuclei
nucleotide sequences
Phylogeny
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
Restriction Mapping
ribosomal DNA
Scutellospora castanea
Sequence Analysis, DNA
Spores, Fungal - genetics
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Summary:Polymorphism of the internal transcribed spacers (ITS) of the ribosomal DNA in Scutellospora castanea (Glomales, Zygomycota) and its organization among spores were evaluated. Polymerase chain reaction (PCR) amplification with ITS1/ITS4 primers yielded several fragments of different lengths, even from single spores. Fragments produced from multisporal DNA were cloned and grouped into 6 ITS types by PCR-RFLP and sequence analysis. Five type-specific primers were designed. Spores were then analyzed by PCR and amplification profiles revealed that they were qualitatively different one from another due to the presence or absence of some ITS types. Intrasporal segregation of ITS variant length types was also shown, by PCR experiments, utilizing diluted fractions of nuclear suspensions from single spores. The results demonstrate the mainly multikaryotic condition of the spores of S. castanea.
ISSN:1087-1845
1096-0937
DOI:10.1006/fgbi.1998.1112