Effect of PLGA as a polymeric emulsifier on preparation of hydrophilic protein-loaded solid lipid nanoparticles

Most proteins are hydrophilic and poorly encapsulated into the hydrophobic matrix of solid lipid nanoparticles (SLN). To solve this problem, poly (lactic-co-glycolic acid) (PLGA) was utilized as a lipophilic polymeric emulsifier to prepare hydrophilic protein-loaded SLN by w/o/w double emulsion and...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2008-12, Vol.67 (2), p.199-204
Main Authors: Xie, ShuYu, Wang, SiLiang, Zhao, BaoKai, Han, Chao, Wang, Ming, Zhou, WenZhong
Format: Article
Language:English
Subjects:
Drug Carriers - chemistry
Emulsification
Emulsifying Agents - chemistry
Hydrophilic protein
Insulin - chemistry
Lactic Acid - chemistry
Lipids - chemistry
Muramidase - chemistry
Nanoparticles - chemistry
Poly (lactic-co-glycolic acid) (PLGA)
Polyglycolic Acid - chemistry
Polymeric emulsifier
Proteins - chemistry
Serum Albumin, Bovine - chemistry
Solid lipid nanoparticles (SLN)
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Summary:Most proteins are hydrophilic and poorly encapsulated into the hydrophobic matrix of solid lipid nanoparticles (SLN). To solve this problem, poly (lactic-co-glycolic acid) (PLGA) was utilized as a lipophilic polymeric emulsifier to prepare hydrophilic protein-loaded SLN by w/o/w double emulsion and solvent evaporation techniques. Hydrogenated castor oil (HCO) was used as a lipid matrix and bovine serum albumin (BSA), lysozyme and insulin were used as model proteins to investigate the effect of PLGA on the formulation of the SLN. The results showed that PLGA was essential for the primary w/o emulsification. In addition, the stability of the w/o emulsion, the encapsulation efficiency and loading capacity of the nanoparticles were enhanced with the increase of PLGA concentration. Furthermore, increasing PLGA concentration decreased zeta potential significantly but had no influence on particle size of the SLN. In vitro release study showed that PLGA significantly affected the initial burst release, i.e. the higher the content of PLGA, the lower the burst release. The released proteins maintained their integrity and bioactivity as confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and biological assay. These results demonstrated that PLGA was an effective emulsifier for the preparation of hydrophilic protein-loaded SLN.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2008.08.018