Specific expression of short‐interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells

RNA interference (RNAi) has been shown to be an effective method for inhibiting the expression of a given gene in human cells by targeting with short duplex RNA (short‐interfering RNA or siRNA). However, more and more studies suggest that non‐specific effects can be induced by siRNAs, such as off‐ta...

Full description

Saved in:
Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2008-11, Vol.40 (11), p.928-933
Main Authors: Luan, Xuejing, Guo, Limin, Yang, Zuozhen, Liu, Min, Li, Xin, Tang, Hua
Format: Article
Language:English
Subjects:
Base Sequence
Cell Line
Cell Line, Tumor
DNA Primers
gene therapy
Genes, Reporter
hTERT promoter
Humans
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA, Small Interfering - genetics
RNAi
short‐interfering RNA
telomerase
Telomerase - genetics
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RNA interference (RNAi) has been shown to be an effective method for inhibiting the expression of a given gene in human cells by targeting with short duplex RNA (short‐interfering RNA or siRNA). However, more and more studies suggest that non‐specific effects can be induced by siRNAs, such as off‐target inhibition, activation of interferon response, and saturation of cellular silencing machinery. It has been known that more than 90% of human tumors exhibit telomerase activity. Consequently, telomerase is believed to be a broadspectrum molecular marker of malignancies. In the present study we attempt to develop a tumor‐specific RNAi system using the human telomerase reverse transcriptase promoter. This system may provide a basis for RNAi therapy.
ISSN:1672-9145
1745-7270
DOI:10.1111/j.1745-7270.2008.00475.x