Anisakis simplex s.l.: Larvicidal activity of various monoterpenic derivatives of natural origin against L3 larvae in vitro and in vivo

• Published in: Experimental parasitology 2008-12, Vol.120 (4), p.295-299
• Main Authors: Navarro, M.C, Noguera, M.A, Romero, M.C, Montilla, M.P, González de Selgas, J.M, Valero, A
• Format: Article
• Language: English
• Subjects: Animals; anisakiasis; Anisakiasis - drug therapy; Anisakiasis - parasitology; Anisakiasis - prevention & control; Anisakis - drug effects; Anisakis simplex; Anisakis simplex sensu lato; Anthelmintics - chemistry; Anthelmintics - pharmacology; Anthelmintics - therapeutic use; Biological and medical sciences; chemical concentration; essential oils; Female; Fish Diseases - drug therapy; Fish Diseases - parasitology; Fish Diseases - prevention & control; Fundamental and applied biological sciences. Psychology; Gadiformes - parasitology; in vitro studies; in vivo studies; Intestines - drug effects; Intestines - parasitology; Larva - drug effects; larvicides; lesions (animal); Life cycle. Host-agent relationship. Pathogenesis; Monoterpenes - chemistry; Monoterpenes - pharmacology; Monoterpenes - therapeutic use; monoterpenoids; mortality; nematicidal properties; nematode larvae; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; Oils, Volatile - therapeutic use; Parasitic Sensitivity Tests; Peritoneal Cavity - parasitology; Protozoa; Rats; Rats, Wistar; Stomach - drug effects; Stomach - parasitology; Stomach - pathology
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2008.07.014

This paper describes the activity against Anisakis simplex s.l. L3 larvae of six monoterpenic derivatives obtained from different essential oils, (α-pinene, β-pinene, ocimene, myrcene, geranyl acetate, and cineole). In in vitro assays, α-pinene, ocimene and cineole showed high activity at a concentration of 125 μg/mL (48 h) but only α-pinene and ocimene were active at 62.5 μg/mL. In in vivo assays, L3 larvae and study compounds were simultaneously administered per os to Wistar rats. The most active compound was α-pinene, finding lesions in only 20% of treated rats versus 98% of controls. Further in vivo studies are required to investigate whether addition of these compounds to food could have a prophylactic effect, reducing the pathogenicity of A. simplex s.l. L3 in humans, and to explore any possible synergy among compounds.