Organization and Chromosomal Mapping of Mouse Gh/Tissue Transglutaminase Gene (Tgm2)

The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis ofTgm2and comparison with the TGase s...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 1999-06, Vol.366 (1), p.151-156
Main Authors: Nanda, Nisha, Iismaa, Siiri E., Copeland, Neal G., Gilbert, Debra J., Jenkins, Nancy, Graham, Robert M., Sutrave, Pramod
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Crosses, Genetic
Exons
Female
gene
Genomic Library
GTP Phosphohydrolases - genetics
GTP-Binding Proteins - genetics
Introns
Male
mapping
Mice
Molecular Sequence Data
Multienzyme Complexes - genetics
organization
Restriction Mapping
sequence
Species Specificity
tissue transglutaminase
Transglutaminases - genetics
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Summary:The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis ofTgm2and comparison with the TGase sequences of other species allowed correction of several apparent sequencing artifacts in theTgm2cDNA.Tgm2spans approximately 34 kb and has 13 exons and 12 introns. Although the structure ofTgm2shows similarity to that of other transglutaminase genes, with introns ranging from 921 bp to >5 kb, several introns differ considerably in size from those of the human Ghgene,TGM2. Tgm2maps to the distal region of mouse chromosome 2, a region syntenic to human chromosome 20q containing TGM2.Tgm2is in the vicinity of two uncloned mouse mutations, diminutive (dm) and blind-sterile (bs). Genomic DNA fromdmmice was unavailable; however, Southern blot analysis ofbsDNA showed no gross rearrangements ofTgm2.
ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.1999.1189