An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads

This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA...

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Bibliographic Details
Published in:Journal of microbiological methods 2008-12, Vol.75 (3), p.576-578
Main Authors: Lim, Crystale Siew Ying, Tung, Chee Hong, Rosli, Rozita, Chong, Pei Pei
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Candida
Candida - chemistry
Candida - genetics
Cell Wall - chemistry
Cell wall disruption
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Fundamental and applied biological sciences. Psychology
Genetic Techniques
Genomic DNA isolation
Glass beads
Microbiology
RNA, Fungal - genetics
RNA, Fungal - isolation & purification
Sorbitol - chemistry
Sorbitol lysis buffer
Total RNA isolation
Yeast
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Summary:This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2008.07.026