Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China

Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strain...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2008-12, Vol.32 (6), p.529-533
Main Authors: Gu, Bing, Pan, Shiyang, Wang, Tong, Zhao, Wenjun, Mei, Yaning, Huang, Peijun, Tong, Mingqing
Format: Article
Language:English
Subjects:
Cassette array
China
Class 1 integron
Class 2 integron
Cross Infection - microbiology
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Enterobacteriaceae
Enterobacteriaceae - genetics
Enterobacteriaceae Infections - microbiology
Genes, Bacterial - genetics
Infectious Disease
Integrons - genetics
Microbial Sensitivity Tests
Polymorphism, Restriction Fragment Length
Resistance
Reverse Transcriptase Polymerase Chain Reaction
Shigella
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Summary:Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strains contained class 1 integrons, and 17 (65.4%) of 26 Shigella contained class 2 integrons. Class 3 integrons were not detected in this study. Restriction fragment length polymorphism (RFLP) and DNA sequencing analysis revealed seven different cassette arrays of class 1 integrons; additionally, four cassette arrays identified in this study were reported for the first time in some species. The genes most commonly found in these class 1 integrons were those for aminoglycoside and trimethoprim resistance. In conclusion, class 1 and 2 integrons were widespread in Enterobacteriaceae and Shigella , respectively, in Nanjing area of China and it was likely that integrons played an important role in antibiotic resistance. Characterisation of cassette arrays of integrons could be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2008.06.019