The polymorphisms of bovine cocaine-and amphetamine-regulated transcripts and their associations with cattle (Bos taurus) growth traits

• Published in: Journal of biosciences 2008-09, Vol.33 (3), p.365-370
• Main Authors: Zhang, Chun Lei, Chen, Hong, Wang, Yan Hong, Lan, Xian Yong, Lei, Chu Zhao, Fang, Xing Tang
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Body weight; Body Weight - genetics; Cattle; Cattle - genetics; Cattle - growth & development; Cell Biology; Cocaine; Gene loci; Genotype; Life Sciences; Methamphetamine; Microbiology; Nerve Tissue Proteins - genetics; Neurotransmitters; Peptides; Physical growth; Plant Sciences; Polymorphism; Polymorphism, Single Nucleotide; Zoology
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-008-0056-1

We investigated the polymorphisms of bovine cocaine-and amphetamine-regulated transcripts ( CART ). The coding and regulating regions of CART were screened in 7 cattle breeds by the single-stranded conformation polymorphism (SSCP) technique. The four loci (C1, C2, C3 and C4) studied were all polymorphic. Polymerase chain reaction (PCR) products representing different SSCP variants were sequenced and a total of 9 single-nucleotide polymorphisms (SNPs) were found. The associations between polymorphic loci and the growth traits of Nanyang cattle were analysed. The results indicated that genotype A 1 A 1 of the C1 locus was associated with a higher body weight ( P < 0.05) than heterozygous A 1 B 1 . Genotype A 2 A 2 of the C2 locus was associated with lower body weight and average daily weight gain ( P ≤ 0.001) than heterozygous A 2 B 2 . C3 and C4 loci had no significant effect on Nanyang cattle growth traits ( P > 0.05).