Peripheral blood T lymphocytes from asthmatic patients are primed for enhanced expression of interleukin (IL)-4 and IL-5 mRNA: associations with lung function and serum IgE

Background The TH2‐like cytokines interleukin (IL)‐4 and IL‐5 play a pivotal role in airway wall inflammation in asthma and these cytokines are increased in peripheral blood and bronchoalveolar lavage fluid from asthmatic patients. It is unclear why specifically TH2‐like cytokines are increased in a...

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Bibliographic Details
Published in:Clinical and experimental allergy 1999-06, Vol.29 (6), p.772-779
Main Authors: BORGER, P, TEN HACKEN, N. H. T, VELLENGA, E, KAUFFMAN, H. F, POSTMA, D. S
Format: Article
Language:English
Subjects:
adenylyl cyclase
Adult
Allergic diseases
asthma
Asthma - blood
Asthma - physiopathology
Biological and medical sciences
Dinoprostone - pharmacology
Female
Humans
Immunoglobulin E - blood
Immunopathology
Interferon-gamma - genetics
interferon-γ
interleukin-4
Interleukin-4 - genetics
interleukin-5
Interleukin-5 - genetics
Lung - physiopathology
Lymphocyte Activation - physiology
Male
Medical sciences
prostaglandin E2
Reference Values
Respiratory and ent allergic diseases
RNA, Messenger - metabolism
T lymphocytes
T-Lymphocytes - metabolism
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Summary:Background The TH2‐like cytokines interleukin (IL)‐4 and IL‐5 play a pivotal role in airway wall inflammation in asthma and these cytokines are increased in peripheral blood and bronchoalveolar lavage fluid from asthmatic patients. It is unclear why specifically TH2‐like cytokines are increased in asthmatic patients. A possible explanation may be an impaired adenylyl cyclase activity, which has been observed in peripheral blood mononuclear cells of asthmatics. Objective To assess interferon (IFN)‐γ, IL‐4 and IL‐5 mRNA expressions and their control by prostaglandin E2 (PGE2), which activates adenylyl cyclases, of peripheral T lymphocytes from patients with moderately severe asthma and healthy controls. Methods Peripheral blood T lymphocytes from asthmatics and healthy controls were isolated and stimulated with antibodies against CD3 plus CD28 in the absence and presence of increasing concentrations of PGE2. IFN‐γ, IL‐4 and IL‐5 mRNA levels were detected by reverse transcription‐polymerase chain reaction. Results In contrast to IFN‐γ mRNA, IL‐4 (P = 0.03, n = 8) and IL‐5 (P 
ISSN:0954-7894
1365-2222
DOI:10.1046/j.1365-2222.1999.00478.x