Identification of a gene encoding the pyruvate decarboxylase gene regulator CaPdc2p from Candida albicans

• Published in: Yeast (Chichester, England) England), 1999-05, Vol.15 (7), p.585-591
• Main Authors: Kaiser, Burkhard, Munder, Thomas, Saluz, Hans‐Peter, Künkel, Waldemar, Eck, Raimund
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Blotting, Southern; Candida albicans; Candida albicans - genetics; Chromosome Mapping; DNA, Fungal - analysis; DNA, Fungal - genetics; Fungal Proteins - chemistry; Fungal Proteins - genetics; Genes, Fungal; Genes, Regulator - genetics; Genetic Complementation Test; Kluyveromyces lactis; Molecular Sequence Data; Polymerase Chain Reaction - methods; pyruvate decarboxylase; Pyruvate Decarboxylase - genetics; Pyruvate Decarboxylase - metabolism; regulatory gene; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sequence Analysis, DNA; Transcription Factors
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/(SICI)1097-0061(199905)15:7<585::AID-YEA401>3.0.CO;2-9

In a screen for Candida albicans genes encoding transactivating proteins, a pyruvate decarboxylase (EC 4.1.1.1.) regulator gene was isolated. An open reading frame (ORF) of 2511 bp was identified encoding a predicted protein of 836 amino acids with a molecular weight of 94·4 kDa. The protein showed glutamine‐ and proline‐rich stretches typical for transcriptional activators. The amino acid sequence comparisons between CaPdc2p of C. albicans and both Pdc2p of Saccharomyces cerevisiae and Rag3p of Kluyveromyces lactis, revealed similarities of 40% and 39%, respectively. The CaPDC2 gene was localized on chromosome 1. Southern blot analysis indicated that CaPDC2 might be a single copy gene. The growth defect of a S. cerevisiae pdc2Δ mutant on glucose was compensated by transformation of the C. albicans CaPDC2 gene. The EMBL Accession No. for the sequence reported in this paper is Y17007. Copyright © 1999 John Wiley & Sons, Ltd.