Phylogenetic evidence for homologous recombination within the family Birnaviridae

School of Biological Sciences, The University of Hong Kong, Hong Kong SAR Correspondence Frederick Chi-Ching Leung fcleung{at}hkucc.hku.hk Birnaviruses are bi-segmented double-stranded RNA (dsRNA) viruses infecting insects, avian species and a wide range of aquatic species. Although homologous recom...

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Bibliographic Details
Published in:Journal of general virology 2008-12, Vol.89 (12), p.3156-3164
Main Authors: Hon, Chung-Chau, Lam, Tommy Tsan-Yuk, Yip, Chi-Wai, Wong, Raymond Tsz-Yeung, Shi, Mang, Jiang, Jingwei, Zeng, Fanya, Leung, Frederick Chi-Ching
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aquabirnavirus - classification
Aquabirnavirus - genetics
Biological and medical sciences
Birnaviridae
Birnaviridae - classification
Birnaviridae - genetics
Databases, Genetic
Fundamental and applied biological sciences. Psychology
Infectious bursal disease virus
Infectious bursal disease virus - classification
Infectious bursal disease virus - genetics
Microbiology
Miscellaneous
Phylogeny
Recombination, Genetic
RNA Replicase - genetics
Sequence Alignment
Sequence Analysis, DNA
Species Specificity
Vaccines, Attenuated
Viral Structural Proteins - genetics
Viral Vaccines
Virology
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Summary:School of Biological Sciences, The University of Hong Kong, Hong Kong SAR Correspondence Frederick Chi-Ching Leung fcleung{at}hkucc.hku.hk Birnaviruses are bi-segmented double-stranded RNA (dsRNA) viruses infecting insects, avian species and a wide range of aquatic species. Although homologous recombination is a common phenomenon in positive-sense RNA viruses, recombination in dsRNA viruses is rarely reported. Here we performed a comprehensive survey on homologous recombination in all available sequences (>1800) of the family Birnaviridae based on phylogenetic incongruence. Although inter-species recombination was not evident, potential intra-species recombination events were detected in aquabirnaviruses and infectious bursal disease virus (IBDV). Eight potential recombination events were identified and the possibility that these events were non-naturally occurring was assessed case by case. Five of the eight events were identified in IBDVs and all of these five events involved live attenuated vaccine strains. This finding suggests that homologous recombination between vaccine and wild-type IBDV strains may have occurred; the potential risk of mass vaccination using live vaccines is discussed. This is the first report of evidence for homologous recombination within the family Birnaviridae . A supplementary figure and supplementary sequence data are available with the online version of this paper.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.2008/004101-0