Expression of Trypsin in Human Cancer Cell Lines and Cancer Tissues and Its Tight Binding to Soluble Form of Alzheimer Amyloid Precursor Protein in Culture

• Published in: Journal of biochemistry (Tokyo) 1999-06, Vol.125 (6), p.1067-1076
• Main Authors: Miyata, Satoshi, Koshikawa, Naohiko, Higashi, Shouichi, Miyagi, Yohei, Nagashima, Yoji, Yanoma, Shunsuke, Kato, Yasumasa, Yasumitsu, Hidetaro, Miyazaki, Kaoru
• Format: Article
• Language: English
• Subjects: Amyloid beta-Protein Precursor - metabolism; Animals; APP; Breast Neoplasms - enzymology; cancer; Colonic Neoplasms - enzymology; Culture Media, Conditioned; Female; Humans; Immunohistochemistry; Mice; Neoplasms - enzymology; Protein Binding; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; Solubility; Stomach Neoplasms - enzymology; Stomach Neoplasms - genetics; trypsin; Trypsin - metabolism; Trypsin Inhibitors - metabolism; Trypsinogen - genetics; Trypsinogen - metabolism; Tumor Cells, Cultured; tumor invasion; zymography
• ISSN: 0021-924X
• DOI: 10.1093/oxfordjournals.jbchem.a022388

It was recently found that overexpression of the trypsin gene in tumor cells stimulates their growth in culture and in nude mice. In the present study, expression of trypsin in various human cancer cell lines and tissues was studied by gelatin zymography and immunoblotting before and after enterokinase treatment and by immunohistochemistry. The analyses showed that many stomach, colon, and breast cancer cell lines secreted trypsinogens-1 and/ or -2, as well as an unidentified serine proteinase of about 70 kDa, into culture medium. Lung cancer cell lines secreted 18- and 19-kDa unidentified trypsin-like proteins. Stomach cancer cell lines frequently secreted active trypsin, suggesting that they produced an endogenous activator of trypsinogen, most likely enterokinase. Active trypsin formed a complex with a soluble form of Alzheimer amyloid precursor protein (sAPP), a Kunitz-type trypsin inhibitor, which was secreted by all cell lines tested. This indicated that sAPP is a primary inhibitor of secreted trypsin. Immunohistochemical analysis showed that tryp-sin(ogen) was frequently expressed at high levels in stomach and colon cancers, but scarcely in breast cancers. In the stomach cancers, the trypsin immunoreactivity was higher in the malignant, non-cohesive type than in the cohesive type. These results support the hypothesis that tumor-derived trypsin is involved in the malignant growth of tumor cells, especially stomach cancer cells.