Expression of CD44 Isoforms on Isolated Bone Marrow Plasma Cells and Peripheral CD19+ B Cells of Patients with Multiple Myeloma and Healthy Individuals

The expression of certain isoforms of CD44 was shown to correlate with aggressiveness and metastatic potential of various tumour types. We analysed the expression of the adhesion molecule CD44 and its variant domains (v6, v7, v7/8, v10) on isolated bone marrow (BM) plasma cells and peripheral blood...

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Bibliographic Details
Published in:Leukemia & lymphoma 1999, Vol.34 (1-2), p.95-103
Main Authors: Schuster-Kolbe, Judith, Ludwig, Heinz, Adolf, GÜNther R., Heider, Karl-Heinz
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antigens, CD19 - biosynthesis
B cells
B-Lymphocytes - metabolism
Bone Marrow Cells - metabolism
CD19
CD44 isoforms
Cell Size - physiology
Female
Humans
Hyaluronan Receptors - biosynthesis
Immunophenotyping
Male
Middle Aged
multiple myeloma
Multiple Myeloma - metabolism
Multiple Myeloma - pathology
plasma cells
Plasma Cells - metabolism
Prognosis
Protein Isoforms - biosynthesis
survival
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Summary:The expression of certain isoforms of CD44 was shown to correlate with aggressiveness and metastatic potential of various tumour types. We analysed the expression of the adhesion molecule CD44 and its variant domains (v6, v7, v7/8, v10) on isolated bone marrow (BM) plasma cells and peripheral blood (PBL) CD19+ B cells of 21 patients with MM and 15 healthy donors. B cells and plasma cells were isolated by immunomagnetic sorting and analysed by two-colour flow cytometry. The expression of CD44 isoforms was significantly higher on PBL B cells of patients with MM than in healthy controls. The elevated expression of CD44 isoforms (v6, v7/8, v10) on PBL B cells correlated with reduced overall survival in MM. CD44 isoforms were more strongly expressed on "larger", activated B cells. Furthermore, CD44 isoforms were found to be simultaneously expressed with CD38hi and CD56 on both, B lymphocytes and plasma cells of patients with MM. The determination of CD44 isoforms on circulating B cells may be helpful in defining prognostically unfavourable subgroups in MM.
ISSN:1042-8194
1029-2403
DOI:10.3109/10428199909083384