Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles

Burkholderia pseudomallei, an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation caus...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 2008-12, Vol.190 (6), p.623-631
Main Authors: Muangsombut, Veerachat, Suparak, Supaporn, Pumirat, Pornpan, Damnin, Suwat, Vattanaviboon, Paiboon, Thongboonkerd, Visith, Korbsrisate, Sunee
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Burkholderia pseudomallei - genetics
Burkholderia pseudomallei - physiology
Cell Fusion
Cytoplasm
Epithelial Cells - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Giant Cells - metabolism
Glycoproteins
HeLa Cells
Humans
Inactivation
Kinases
Macrophages - metabolism
Melioidosis - microbiology
Mice
Microbiology
Miscellaneous
Mutation
Proteins
R&D
Research & development
Salmonella
Transport Vesicles - metabolism
Transport Vesicles - microbiology
Vacuoles - metabolism
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Burkholderia pseudomallei, an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation caused a marked decrease in secretion of BopE effector and BipD translocator proteins into culture supernatant. The B. pseudomallei bsaQ mutant also exhibited decreased efficiencies of plaque formation, invasion into non-phagocytic cells and multinucleated giant cell (MNGC) development in a J774A.1 macrophage cell line. Co-localization of the bacteria and lysosome-associated membrane glycoprotein-1 (LAMP-1) containing vesicles suggested that defects in MNGC formation may result from the delayed ability of this B. pseudomallei mutant to escape from the vacuoles of macrophages.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-008-0413-3