A novel 45 kDa secretory protein from rat olfactory epithelium: primary structure and localisation

cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46 026 Da. A homology was revealed between the amino acid sequence of...

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Bibliographic Details
Published in:FEBS letters 1999-04, Vol.450 (1), p.126-130
Main Authors: Merkulova, Maria I., Andreeva, Svetlana G., Shuvaeva, Tatyana M., Novoselov, Sergey V., Peshenko, Igor V., Bystrova, Marina F., Novoselov, Vladimir I., Fesenko, Evgeny E., Lipkin, Valery M.
Format: Article
Language:English
Subjects:
45 kDa secretory protein
Amino Acid Sequence
Animals
Base Sequence
cDNA cloning
Cloning, Molecular
DNA, Complementary - chemistry
In Situ Hybridization
Molecular Sequence Data
Olfactory epithelium
Olfactory Mucosa - chemistry
Proteins - chemistry
Proteins - genetics
Rats
Restriction Mapping
RNA, Messenger - analysis
Sequence Alignment
Transfer protein
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Summary:cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46 026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein and the proteins involved in the transfer of hydrophobic ligands. Using in situ hybridisation, the 45 kDa protein mRNA expression was detected in the layer of supportive cells of olfactory epithelium, apical region of trachea, surface layer of the ciliated bronchial epithelium in lung and in skin epidermis.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)00470-6