Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1

• Published in: Journal of general virology 1999-05, Vol.80 (5), p.1263-1269
• Main Authors: Zakhartchouk, AN, Pyne, C, Mutwiri, GK, Papp, Z, Baca-Estrada, ME, Griebel, P, Babiuk, LA, Tikoo, SK
• Format: Article
• Language: English
• Subjects: Adenovirus E3 Proteins - genetics; Adenovirus E3 Proteins - immunology; Animals; Antibodies, Viral - blood; Antigens, Viral - immunology; Bovine adenovirus 3; Bovine herpesvirus 1; Cattle; Cattle Diseases - immunology; Cattle Diseases - prevention & control; Genetic Vectors; Herpesviridae Infections - immunology; Herpesviridae Infections - prevention & control; Herpesviridae Infections - veterinary; Herpesvirus 1, Bovine - immunology; Immunity, Mucosal; Immunization; Mastadenovirus - genetics; Mastadenovirus - immunology; Recombinant Proteins - immunology; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - immunology; Viral Proteins - immunology; Viral Vaccines - administration & dosage; Viral Vaccines - immunology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-80-5-1263

AN Zakhartchouk, C Pyne, GK Mutwiri, Z Papp, ME Baca-Estrada, P Griebel, LA Babiuk and SK Tikoo Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3 To determine the potential of replication-competent (E3-deleted) bovine adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves, we evaluated the ability of recombinant BAV-3 expressing different forms of of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1 infection in calves that had pre-existing BAV-3 specific antibodies. Three- to four-month-old calves, vaccinated intranasally with recombinant BAV-3 expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt) (BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced gD-specific antibody responses in serum and nasal secretions, and primed calves for gD-specific lymphoproliferative responses. In addition, all calves developed complement-independent neutralizing antibodies against BHV-1. Protection against viral challenge was observed in calves vaccinated with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant reduction in body temperature and clinical disease, and a partial reduction in the amount and duration of virus excretion in nasal secretions. These results indicate that replication-competent BAV-3-based vectors can induce protective immune responses in calves (the natural host) that have pre-existing BAV-3-specific antibodies.