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Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1
AN Zakhartchouk, C Pyne, GK Mutwiri, Z Papp, ME Baca-Estrada, P Griebel, LA Babiuk and SK Tikoo Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3 To determine the potential of replication-competent (E3-deleted) bovine adenovirus-3...
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| Published in: | Journal of general virology 1999-05, Vol.80 (5), p.1263-1269 |
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| container_title | Journal of general virology |
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| creator | Zakhartchouk, AN Pyne, C Mutwiri, GK Papp, Z Baca-Estrada, ME Griebel, P Babiuk, LA Tikoo, SK |
| description | AN Zakhartchouk, C Pyne, GK Mutwiri, Z Papp, ME Baca-Estrada, P Griebel, LA Babiuk and SK Tikoo
Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3
To determine the potential of replication-competent (E3-deleted) bovine
adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves,
we evaluated the ability of recombinant BAV-3 expressing different forms of
of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1
infection in calves that had pre-existing BAV-3 specific antibodies. Three-
to four-month-old calves, vaccinated intranasally with recombinant BAV-3
expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt)
(BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged
with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced
gD-specific antibody responses in serum and nasal secretions, and primed
calves for gD-specific lymphoproliferative responses. In addition, all
calves developed complement-independent neutralizing antibodies against
BHV-1. Protection against viral challenge was observed in calves vaccinated
with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant
reduction in body temperature and clinical disease, and a partial reduction
in the amount and duration of virus excretion in nasal secretions. These
results indicate that replication-competent BAV-3-based vectors can induce
protective immune responses in calves (the natural host) that have
pre-existing BAV-3-specific antibodies. |
| doi_str_mv | 10.1099/0022-1317-80-5-1263 |
| format | article |
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Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3
To determine the potential of replication-competent (E3-deleted) bovine
adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves,
we evaluated the ability of recombinant BAV-3 expressing different forms of
of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1
infection in calves that had pre-existing BAV-3 specific antibodies. Three-
to four-month-old calves, vaccinated intranasally with recombinant BAV-3
expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt)
(BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged
with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced
gD-specific antibody responses in serum and nasal secretions, and primed
calves for gD-specific lymphoproliferative responses. In addition, all
calves developed complement-independent neutralizing antibodies against
BHV-1. Protection against viral challenge was observed in calves vaccinated
with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant
reduction in body temperature and clinical disease, and a partial reduction
in the amount and duration of virus excretion in nasal secretions. These
results indicate that replication-competent BAV-3-based vectors can induce
protective immune responses in calves (the natural host) that have
pre-existing BAV-3-specific antibodies.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-80-5-1263</identifier><identifier>PMID: 10355773</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Adenovirus E3 Proteins - genetics ; Adenovirus E3 Proteins - immunology ; Animals ; Antibodies, Viral - blood ; Antigens, Viral - immunology ; Bovine adenovirus 3 ; Bovine herpesvirus 1 ; Cattle ; Cattle Diseases - immunology ; Cattle Diseases - prevention & control ; Genetic Vectors ; Herpesviridae Infections - immunology ; Herpesviridae Infections - prevention & control ; Herpesviridae Infections - veterinary ; Herpesvirus 1, Bovine - immunology ; Immunity, Mucosal ; Immunization ; Mastadenovirus - genetics ; Mastadenovirus - immunology ; Recombinant Proteins - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology ; Viral Proteins - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology</subject><ispartof>Journal of general virology, 1999-05, Vol.80 (5), p.1263-1269</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-c3c388310b7f6753ecb2844a040fed463f53c636c8cce21e10bc95c8ff409faa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10355773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zakhartchouk, AN</creatorcontrib><creatorcontrib>Pyne, C</creatorcontrib><creatorcontrib>Mutwiri, GK</creatorcontrib><creatorcontrib>Papp, Z</creatorcontrib><creatorcontrib>Baca-Estrada, ME</creatorcontrib><creatorcontrib>Griebel, P</creatorcontrib><creatorcontrib>Babiuk, LA</creatorcontrib><creatorcontrib>Tikoo, SK</creatorcontrib><title>Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>AN Zakhartchouk, C Pyne, GK Mutwiri, Z Papp, ME Baca-Estrada, P Griebel, LA Babiuk and SK Tikoo
Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3
To determine the potential of replication-competent (E3-deleted) bovine
adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves,
we evaluated the ability of recombinant BAV-3 expressing different forms of
of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1
infection in calves that had pre-existing BAV-3 specific antibodies. Three-
to four-month-old calves, vaccinated intranasally with recombinant BAV-3
expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt)
(BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged
with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced
gD-specific antibody responses in serum and nasal secretions, and primed
calves for gD-specific lymphoproliferative responses. In addition, all
calves developed complement-independent neutralizing antibodies against
BHV-1. Protection against viral challenge was observed in calves vaccinated
with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant
reduction in body temperature and clinical disease, and a partial reduction
in the amount and duration of virus excretion in nasal secretions. These
results indicate that replication-competent BAV-3-based vectors can induce
protective immune responses in calves (the natural host) that have
pre-existing BAV-3-specific antibodies.</description><subject>Adenovirus E3 Proteins - genetics</subject><subject>Adenovirus E3 Proteins - immunology</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens, Viral - immunology</subject><subject>Bovine adenovirus 3</subject><subject>Bovine herpesvirus 1</subject><subject>Cattle</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - prevention & control</subject><subject>Genetic Vectors</subject><subject>Herpesviridae Infections - immunology</subject><subject>Herpesviridae Infections - prevention & control</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesvirus 1, Bovine - immunology</subject><subject>Immunity, Mucosal</subject><subject>Immunization</subject><subject>Mastadenovirus - genetics</subject><subject>Mastadenovirus - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkU9vFSEUxYnR2Gf1EzQxrEw3VBj-DOPONK1tUuNG14ThXfpoZuAVZl5TE7-7vMyr7c4Vl9zfOZBzEDph9IzRrvtMadMQxllLNCWSsEbxV2jFhJKkqfvXaPWPOELvSrmjlAkh27foiFEuZdvyFfrzfXap2AGHcZxj-G2nkCJOHjs77KDghzBtcAaXxj5EGyfcp12IgO0aYp3yXAj_gkNcz-5Juc1pgnrbwcF0esRTehJuIG-hLEr2Hr3xdijw4XAeo1-XFz_Pr8jNj2_X519viBOMTsRxx7XmjPatV63k4PpGC2GpoB7WQnEvuVNcOe0cNAwq6DrptPeCdt5afow-Lb71b_czlMmMoTgYBhshzcWoru24Uvq_IGs504rSCvIFdDmVksGbbQ6jzY-GUbOvx-zDN_vwjaZGmn09VfXxYD_3I6xfaJY-KnC6AJtwu3kIGcwtxDHUR_qQTE3t2esvTmObzA</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Zakhartchouk, AN</creator><creator>Pyne, C</creator><creator>Mutwiri, GK</creator><creator>Papp, Z</creator><creator>Baca-Estrada, ME</creator><creator>Griebel, P</creator><creator>Babiuk, LA</creator><creator>Tikoo, SK</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1</title><author>Zakhartchouk, AN ; 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Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada S7N 5E3
To determine the potential of replication-competent (E3-deleted) bovine
adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves,
we evaluated the ability of recombinant BAV-3 expressing different forms of
of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1
infection in calves that had pre-existing BAV-3 specific antibodies. Three-
to four-month-old calves, vaccinated intranasally with recombinant BAV-3
expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt)
(BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged
with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced
gD-specific antibody responses in serum and nasal secretions, and primed
calves for gD-specific lymphoproliferative responses. In addition, all
calves developed complement-independent neutralizing antibodies against
BHV-1. Protection against viral challenge was observed in calves vaccinated
with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant
reduction in body temperature and clinical disease, and a partial reduction
in the amount and duration of virus excretion in nasal secretions. These
results indicate that replication-competent BAV-3-based vectors can induce
protective immune responses in calves (the natural host) that have
pre-existing BAV-3-specific antibodies.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>10355773</pmid><doi>10.1099/0022-1317-80-5-1263</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1999-05, Vol.80 (5), p.1263-1269 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69793668 |
| source | Freely Accessible Science Journals |
| subjects | Adenovirus E3 Proteins - genetics Adenovirus E3 Proteins - immunology Animals Antibodies, Viral - blood Antigens, Viral - immunology Bovine adenovirus 3 Bovine herpesvirus 1 Cattle Cattle Diseases - immunology Cattle Diseases - prevention & control Genetic Vectors Herpesviridae Infections - immunology Herpesviridae Infections - prevention & control Herpesviridae Infections - veterinary Herpesvirus 1, Bovine - immunology Immunity, Mucosal Immunization Mastadenovirus - genetics Mastadenovirus - immunology Recombinant Proteins - immunology Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology Viral Proteins - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology |
| title | Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1 |
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