Induction of Genital Immunity by DNA Priming and Intranasal Booster Immunization with a Replication-Defective Adenoviral Recombinant

Mice immunized through different routes such as i.m., intradermally, or intratracheally with a DNA vaccine to rabies virus developed high titers of serum Ab but only borderline levels of mucosal Abs determined from vaginal secretions. DNA vaccines given by either route enhanced vaginal IgA and IgG2a...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1999-06, Vol.162 (11), p.6716-6723
Main Authors: Xiang, Zhi Quan, Pasquini, Susanna, Ertl, Hildegund C. J
Format: Article
Language:English
Subjects:
Adenovirus
Adenoviruses, Human - genetics
Adenoviruses, Human - immunology
Administration, Intranasal
Animals
Antibodies, Viral - biosynthesis
Antigens, Viral - biosynthesis
Antigens, Viral - genetics
Defective Viruses - genetics
Defective Viruses - immunology
Female
Genetic Vectors - administration & dosage
Genetic Vectors - immunology
Immunity, Mucosal - genetics
Immunity, Mucosal - immunology
Immunization, Secondary - methods
Injections, Intradermal
Injections, Intramuscular
Interleukin-4 - administration & dosage
Interleukin-5 - administration & dosage
Mice
Mice, Inbred C3H
Rabies Vaccines - genetics
Rabies Vaccines - immunology
Rabies virus
Recombination, Genetic - immunology
Vaccines, Combined - genetics
Vaccines, Combined - immunology
Vaccines, DNA - immunology
Vagina - immunology
Viral Vaccines - genetics
Viral Vaccines - immunology
Virus Replication - genetics
Virus Replication - immunology
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Description
Summary:Mice immunized through different routes such as i.m., intradermally, or intratracheally with a DNA vaccine to rabies virus developed high titers of serum Ab but only borderline levels of mucosal Abs determined from vaginal secretions. DNA vaccines given by either route enhanced vaginal IgA and IgG2a secretion upon a subsequent intranasal booster immunization with an E1-deleted adenoviral recombinant expressing the same Ag of rabies virus. DNA vaccine priming reduced the Ab response to the adenoviral Ags and counterbalanced the impaired B cell response to the rabies virus Ag expressed by the adenoviral recombinant in mice preimmune to adenovirus. The vaginal B cell response could further be enhanced by using the Th2-type cytokines IL-4 or IL-5 as genetic adjuvants concomitantly with the DNA vaccine before intranasal booster immunization with the recombinant vaccine.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.162.11.6716