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Induction of Genital Immunity by DNA Priming and Intranasal Booster Immunization with a Replication-Defective Adenoviral Recombinant
Mice immunized through different routes such as i.m., intradermally, or intratracheally with a DNA vaccine to rabies virus developed high titers of serum Ab but only borderline levels of mucosal Abs determined from vaginal secretions. DNA vaccines given by either route enhanced vaginal IgA and IgG2a...
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| Published in: | The Journal of immunology (1950) 1999-06, Vol.162 (11), p.6716-6723 |
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| cites | cdi_FETCH-LOGICAL-c413t-46d5de7da6ab1255a8154cb1c20f1800a8a2f7298f748e64ddd0e88be297c1f3 |
| container_end_page | 6723 |
| container_issue | 11 |
| container_start_page | 6716 |
| container_title | The Journal of immunology (1950) |
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| creator | Xiang, Zhi Quan Pasquini, Susanna Ertl, Hildegund C. J |
| description | Mice immunized through different routes such as i.m., intradermally, or intratracheally with a DNA vaccine to rabies virus developed high titers of serum Ab but only borderline levels of mucosal Abs determined from vaginal secretions. DNA vaccines given by either route enhanced vaginal IgA and IgG2a secretion upon a subsequent intranasal booster immunization with an E1-deleted adenoviral recombinant expressing the same Ag of rabies virus. DNA vaccine priming reduced the Ab response to the adenoviral Ags and counterbalanced the impaired B cell response to the rabies virus Ag expressed by the adenoviral recombinant in mice preimmune to adenovirus. The vaginal B cell response could further be enhanced by using the Th2-type cytokines IL-4 or IL-5 as genetic adjuvants concomitantly with the DNA vaccine before intranasal booster immunization with the recombinant vaccine. |
| doi_str_mv | 10.4049/jimmunol.162.11.6716 |
| format | article |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1999-06, Vol.162 (11), p.6716-6723 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| subjects | Adenovirus Adenoviruses, Human - genetics Adenoviruses, Human - immunology Administration, Intranasal Animals Antibodies, Viral - biosynthesis Antigens, Viral - biosynthesis Antigens, Viral - genetics Defective Viruses - genetics Defective Viruses - immunology Female Genetic Vectors - administration & dosage Genetic Vectors - immunology Immunity, Mucosal - genetics Immunity, Mucosal - immunology Immunization, Secondary - methods Injections, Intradermal Injections, Intramuscular Interleukin-4 - administration & dosage Interleukin-5 - administration & dosage Mice Mice, Inbred C3H Rabies Vaccines - genetics Rabies Vaccines - immunology Rabies virus Recombination, Genetic - immunology Vaccines, Combined - genetics Vaccines, Combined - immunology Vaccines, DNA - immunology Vagina - immunology Viral Vaccines - genetics Viral Vaccines - immunology Virus Replication - genetics Virus Replication - immunology |
| title | Induction of Genital Immunity by DNA Priming and Intranasal Booster Immunization with a Replication-Defective Adenoviral Recombinant |
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