Prostaglandin F(2alpha) negatively regulates bone resorption in murine osteoclast development

Prostaglandin (PG) E(2) promotes osteoclastic cell differentiation, but the physiological function of PGF(2alpha) remains unclear. We examined the physiological effects of PGF(2alpha) on osteoclast differentiation using a murine cell line, RAW264, and the column-purified murine bone marrow cells, bo...

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Bibliographic Details
Published in:Prostaglandins & other lipid mediators 2008-12, Vol.87 (1-4), p.26-33
Main Authors: Kamon, M, Fujita, D, Goto, N, Amano, H, Sakamoto, K
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - drug effects
Bone Marrow Cells - metabolism
Bone Resorption - metabolism
Cell Differentiation
Cell Line
Cell Nucleus - metabolism
Dinoprost - pharmacology
Mice
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Receptors, Prostaglandin - metabolism
Signal Transduction - drug effects
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Summary:Prostaglandin (PG) E(2) promotes osteoclastic cell differentiation, but the physiological function of PGF(2alpha) remains unclear. We examined the physiological effects of PGF(2alpha) on osteoclast differentiation using a murine cell line, RAW264, and the column-purified murine bone marrow cells, both of which are differentiable into osteoclast-like multi-nuclear cells. Although PGF(2alpha) did not affect the number of differentiated osteoclasts, PGF(2alpha) reduced the bone resorption activity of osteoclasts developed from both cell types in a pit formation assay. Thus, PGF(2alpha) inhibits bone resorption without affecting the number of osteoclasts, providing a novel molecular mechanism underlying bone metabolism.
ISSN:1098-8823
DOI:10.1016/j.prostaglandins.2008.06.002