Establishment of two glioma cell lines from two surgical specimens obtained at different times from the same individual

We established two glioma cell lines from two surgical specimens obtained at different times from the same patient. One (No. 9R), which was derived from the recurrent tumor (glioblastoma, grade IV), proliferated more rapidly in vitro than the other (No. 9) from the primary tumor (slightly anaplastic...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuro-oncology 1999-02, Vol.41 (3), p.247-254
Main Authors: ONDA, K, NAGAI, S, TANAKA, R, MORII, K, YOSHIMURA, J.-I, TSUMANUMA, I, KUMANISHI, T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Brain Neoplasms - surgery
Cell Culture Techniques - methods
Cell Cycle Proteins
Cell Division
Cyclin-Dependent Kinase Inhibitor p15
Cyclin-Dependent Kinase Inhibitor p16 - genetics
Gene Deletion
Genes, Tumor Suppressor
Glioma - genetics
Glioma - pathology
Glioma - surgery
Humans
Kinetics
Male
Medical sciences
Mice
Mice, Nude
Middle Aged
Neurology
Time Factors
Transcription Factors - genetics
Transplantation, Heterologous
Tumor Cells, Cultured
Tumor Suppressor Proteins
Tumors of the nervous system. Phacomatoses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We established two glioma cell lines from two surgical specimens obtained at different times from the same patient. One (No. 9R), which was derived from the recurrent tumor (glioblastoma, grade IV), proliferated more rapidly in vitro than the other (No. 9) from the primary tumor (slightly anaplastic astrocytoma, grade II-III). No. 9R showed heterotransplantability in nude mice, whereas No. 9 did not. These findings indicate that No. 9R has a more aggressive or malignant nature than No. 9. Both cell lines showed homozygous deletion of the representative tumor suppressor p16 and p15 genes, but no p53 gene alteration. However, examination of the overall mRNA expression profile using a commercially available cDNA-spotted membrane revealed much higher expression levels of several mRNAs, at least, in No. 9R than in No. 9, although the relationship between these mRNAs and the growth potentials remained unknown. These two cell lines, derived from the same individual, with different proliferating potentials may be useful for studies on the molecular bases of glioma malignancy and progression.
ISSN:0167-594X
1573-7373
DOI:10.1023/A:1006172608019