Trans-10, cis-12-conjugated linoleic acid does not increase body fat loss induced by energy restriction

• Published in: British journal of nutrition 2008-12, Vol.100 (6), p.1245-1250
• Main Authors: Lasa, Arrate, Churruca, Itziar, Simón, Edurne, Fernández-Quintela, Alfredo, Rodríguez, Victor Manuel, Portillo, María Puy
• Format: Article
• Language: English
• Subjects: Adenosine; Adipocytes; Adipocytes - drug effects; Adipose tissue; Adipose Tissue - anatomy & histology; Adipose Tissue - drug effects; Adipose Tissue - enzymology; Adiposity - drug effects; animal models; Animals; Biological and medical sciences; Body fat; Body weight; Caloric Restriction; Cell Size - drug effects; Cellulose; Conjugated linoleic acid; Cricetinae; Diet; Drug Evaluation, Preclinical - methods; Energy; Energy restriction; enzyme activity; Enzymes; Fatty acids; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Glycerol; Hamsters; high fat diet; Kinases; Laboratory animals; Linoleic Acids, Conjugated - pharmacology; Lipase - metabolism; lipid metabolism; Lipids; Lipolysis; Lipolysis - drug effects; Lipoproteins; low calorie diet; Male; Mesocricetus; nutrition physiology; restricted feeding; Seeds; trans-10,cis-12-conjugated linoleic acid; triacylglycerol lipase; Vegetable oils; Vertebrates: anatomy and physiology, studies on body, several organs or systems; weight loss; Weight Loss - drug effects
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S000711450800305X

Very little evidence exists concerning the effects of conjugated linoleic acid (CLA) on body fat reduction induced by energy restriction. Moreover, although an effect of trans-10, cis-12-CLA on lipolysis has been suggested, it has not been consistently shown. The aims of the present study were to determine whether trans-10, cis-12-CLA increases the reduction of body fat induced by energy restriction, and to analyse its effect on lipolysis and adipose tissue lipase expression (hormone-sensitive lipase (HSL) and adipose tissue TAG lipase (ATGL)). Male Syrian Golden hamsters were fed a high-fat diet during 7 weeks in order to make them fatter. Then they were submitted to a mild energy restriction (25 %) without or with supplementation of 0·5 % trans-10, cis-12-CLA for 3 weeks. Basal glycerol release and lipolysis stimulated by several drugs acting at different levels of the lipolytic cascade were measured in epididymal adipose tissue. The expression of HSL and ATGL was assessed by real-time RT-PCR. No differences were found in adipose tissues size between the experimental groups. Medium adipocyte size and total number of adipocytes were similar in both experimental groups. Animals fed the CLA-enriched diet showed similar lipolytic rates as well as HSL and ATGL expressions to the controls. In conclusion, trans-10, cis-12-CLA does not promote adipose tissue lipid mobilisation nor does it heighten body fat reduction induced by energy restriction. Consequently, this CLA isomer does not seem to be a useful tool to be included in body weight-loss strategies followed in obesity treatment.