Changes induced by sucrose administration upon the morphology and function of pancreatic islets in the normal hamster

Background This report documents sequential changes in islet morphology (cell replication and islet neogenesis) and glucose‐induced insulin secretion in young normal male Syrian hamsters. Methods Three‐week‐old animals received a control standard commercial diet or this diet supplemented with sucros...

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Bibliographic Details
Published in:Diabetes/metabolism research and reviews 1999-03, Vol.15 (2), p.106-112
Main Authors: Del Zotto, Héctor, Massa, Laura, Gómez Dumm, César L., Gagliardino, Juan J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood Glucose - metabolism
Body Weight
Cell Division - drug effects
Cricetinae
cytokeratins
Diabetes. Impaired glucose tolerance
Dietary Sucrose - pharmacology
Drinking Behavior
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Energy Intake
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Glucose - pharmacology
In Vitro Techniques
Insulin - blood
Insulin - metabolism
Insulin Secretion
islet function
islet neogenesis
islet-cell replication rate
Islets of Langerhans - cytology
Islets of Langerhans - drug effects
Islets of Langerhans - physiology
Male
Medical sciences
Mesocricetus
sucrose-rich diet
Syrian hamsters
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Summary:Background This report documents sequential changes in islet morphology (cell replication and islet neogenesis) and glucose‐induced insulin secretion in young normal male Syrian hamsters. Methods Three‐week‐old animals received a control standard commercial diet or this diet supplemented with sucrose – 10% (w/v) solution in drinking water, a treatment that stimulated pancreatic growth and function – for 5 (C5/S5) or 21 (C21/S21) weeks. Insulin secretion and content were measured in isolated islets, while several biochemical parameters were assessed in serum. Different morphological features were analysed in the endocrine pancreas by quantitative immunocytochemistry. Results Serum glucose, triglycerides and total cholesterol levels were comparable among the groups, whereas serum‐ and pancreatic‐insulin levels were higher in the S hamsters. Islets from S21 hamsters released more insulin than those from C21 animals at all glucose concentrations tested. The volume densities of the total endocrine pancreas (1.9±0.2 vs 1.2±0.2; p
ISSN:1520-7552
1520-7560
DOI:10.1002/(SICI)1520-7560(199903/04)15:2<106::AID-DMRR18>3.0.CO;2-2