Colony-forming cells in rat myocardium after destructive exposure and intramyocardial transplantation of bone marrow cells

• Published in: Bulletin of experimental biology and medicine 2008, Vol.145 (1), p.137-140
• Main Authors: Afanas’yev, S. A., Sviridov, I. N., Shakhov, V. P., Falaleyeva, L. P., Popov, S. V., Karpov, R. S.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Bone Marrow Cells - cytology; Bone Marrow Transplantation; Cell Biology; Cells, Cultured; Cold Temperature - adverse effects; Heart Ventricles - cytology; Internal Medicine; Laboratory Medicine; Male; Myocardium - cytology; Myocardium - pathology; Myocytes, Cardiac - cytology; Myocytes, Cardiac - physiology; Pathology; Rats; Rats, Wistar; Regeneration - physiology; Stem Cells; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-008-0027-4

The content of colony-forming cells in myocardial cell culture from the perinecrotic zone of rat heart was evaluated on day 40 after cryodestruction. The mean cellularity after cryodestruction was 12-fold lower than in intact animals. Intramyocardial transplantation of bone marrow cells (mononuclears, mesenchymal stem cells, and mesenchymal stem cells treated with 5-azacitidine) into the perinecrotic zone increased the content of colony-forming cells. After transplantation of mesenchymal stem cells and mesenchymal stem cells treated with 5-azacitidine, the number of colonies reached 33±7 and 11±3, the mean cellularity being 2975±80 and 1105±42 cells/cm 2 , respectively. Hence, intramyocardial transplantation of mesenchymal stem cells created an appreciable pool of colony-forming cells in the myocardial perinecrotic zone. Treatment with 5-azacitidine reduced survival of mesenchymal stem cells after intramyocardial transplantation.