Increased interaction of proteins in nuclear extract from mouse liver and lung tumors with TATA-containing oligonucleotide

Increased TATA-binding activity of proteins in nuclear extracts from murine hepatocarcinoma HA-1 and murine Lewis lung adenocarcinoma was demonstrated. The dependence of the amount of formed complexes on protein concentration, displacement of labeled 32 P-TATA-containing oligonucleotide by its unlab...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2008, Vol.145 (1), p.93-95
Main Authors: Lysova, M. V., Arshinov, T. V., Drachkova, I. A., Kobzev, V. F., Kaledin, V. I., Savinkova, L. K.
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Internal Medicine
Laboratory Medicine
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Mice
Mice, Inbred C57BL
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Oligonucleotides - genetics
Oligonucleotides - metabolism
Pathology
TATA Box
TATA-Box Binding Protein - genetics
TATA-Box Binding Protein - metabolism
Tissue Extracts - chemistry
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Summary:Increased TATA-binding activity of proteins in nuclear extracts from murine hepatocarcinoma HA-1 and murine Lewis lung adenocarcinoma was demonstrated. The dependence of the amount of formed complexes on protein concentration, displacement of labeled 32 P-TATA-containing oligonucleotide by its unlabeled analog, and weak interaction with an oligonucleotide containing damaged TATA box confirm specificity of the formed complexes.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-008-0037-2