Candida albicans exoglucanase as a reporter gene in Schizosaccharomyces pombe

The Candida albicans XOG1 gene, previously shown to be a good reporter gene in Saccharomyces cerevisiae and C. albicans, was tested in Schizosaccharomyces pombe. Unlike the budding yeast, S. pombe does not produce exoglucanase activity and hence this system would be applicable to any given strain of...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1999-06, Vol.175 (1), p.143-148
Main Authors: Molero, G, Cid, V.J, Vivar, C, Nombela, C, Sanchez-Perez, M
Format: Article
Language:English
Subjects:
Baking yeast
beta-Glucosidase - genetics
beta-Glucosidase - metabolism
Biological and medical sciences
Biotechnology
Candida albicans
Candida albicans - enzymology
Candida albicans - genetics
Candida albicans exoglucanase
Culture Media
enzyme activity
Flow Cytometry
Fundamental and applied biological sciences. Psychology
gene expression
Genes, Fungal
Genes, Reporter - genetics
Genetic engineering
Genetic technics
Glucan 1,3-beta-Glucosidase
High temperature
Methods. Procedures. Technologies
Microbiology
Miscellaneous
Mycology
Promoter Regions, Genetic
Reporter gene
Saccharomyces cerevisiae
Schizosaccharomyces - enzymology
Schizosaccharomyces - genetics
Schizosaccharomyces - growth & development
Schizosaccharomyces pombe
Synthetic digonucleotides and genes. Sequencing
Temperature
Thiamine - metabolism
Transformation, Genetic
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
xog1 gene
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Candida albicans XOG1 gene, previously shown to be a good reporter gene in Saccharomyces cerevisiae and C. albicans, was tested in Schizosaccharomyces pombe. Unlike the budding yeast, S. pombe does not produce exoglucanase activity and hence this system would be applicable to any given strain of this organism. The XOG1 gene was located under the control of the nmt1 promoter and its functionality could be demonstrated even at high temperatures (37 degrees C). The exoglucanase activity can be measured both in vivo and in vitro by either a simple biochemical reaction (on cells or media) or by flow cytometry, because the cells remain viable after the assay.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13613.x