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Glossogin, a novel phenylpropanoid from Glossogyne tenuifolia, induced apoptosis in A549 lung cancer cells

Glossogyne tenuifolia has been shown to exhibit good antioxidant and anticancer activity. In this study, a new phenylpropanoid compound, glossogin (1′-acetoxy-4- O-isovalyryleugenol), was isolated from ethyl acetate extract of G. tenuifolia by using column chromatography and HPLC. Its chemical struc...

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Published in:Food and chemical toxicology 2008-12, Vol.46 (12), p.3785-3791
Main Authors: Hsu, Hsia-Fen, Houng, Jer-Yiing, Kuo, Chih-Feng, Tsao, Nina, Wu, Yang-Chang
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description Glossogyne tenuifolia has been shown to exhibit good antioxidant and anticancer activity. In this study, a new phenylpropanoid compound, glossogin (1′-acetoxy-4- O-isovalyryleugenol), was isolated from ethyl acetate extract of G. tenuifolia by using column chromatography and HPLC. Its chemical structure was determined by 1H and 13C NMR, MS and IR spectroscopic evidence. This compound showed the cytotoxicity against A549 human lung cancer cell line and it induced the progressing apoptosis on A549 cells. This apoptosis was verified as A549 cells were arrested at the sub-G 1 phase. The apoptosis was accompanied by release of cytochrome C and activation of caspase-9 and -3. It was also associated with the decrease in Bcl-2 and Bcl-xL protein levels, and the increase in Bad protein expression. Data analysis suggests glossogin exerted significant apoptotic effect on A549 cells through the mitochondrial pathway. Hence, our findings showed that glossogin exhibited potential anticancer activity against lung cancer through proliferating inhibition and apoptosis induction of cancer cells.
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In this study, a new phenylpropanoid compound, glossogin (1′-acetoxy-4- O-isovalyryleugenol), was isolated from ethyl acetate extract of G. tenuifolia by using column chromatography and HPLC. Its chemical structure was determined by 1H and 13C NMR, MS and IR spectroscopic evidence. This compound showed the cytotoxicity against A549 human lung cancer cell line and it induced the progressing apoptosis on A549 cells. This apoptosis was verified as A549 cells were arrested at the sub-G 1 phase. The apoptosis was accompanied by release of cytochrome C and activation of caspase-9 and -3. It was also associated with the decrease in Bcl-2 and Bcl-xL protein levels, and the increase in Bad protein expression. Data analysis suggests glossogin exerted significant apoptotic effect on A549 cells through the mitochondrial pathway. 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subjects A549 lung cancer cell
anticarcinogenic activity
Antineoplastic Agents, Phytogenic
antioxidant activity
antioxidants
Apoptosis
Apoptosis - drug effects
Asteraceae - chemistry
Biological and medical sciences
Blotting, Western
Caspases - metabolism
Cell Cycle - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
chemoprevention
cytochrome c
Cytochromes c - metabolism
Cytotoxicity
Drug Screening Assays, Antitumor
Enzyme Activation - drug effects
Flow Cytometry
free radical scavengers
Glossogin
Glossogyne tenuifolia
herbs
Humans
lung neoplasms
Lung Neoplasms - drug therapy
Lung Neoplasms - pathology
Magnetic Resonance Spectroscopy
Mass Spectrometry
Medical sciences
medicinal plants
Mitochondria - drug effects
perennials
plant extracts
Pneumology
Spectrophotometry, Infrared
structure-activity relationships
Toxicology
Tumors of the respiratory system and mediastinum
title Glossogin, a novel phenylpropanoid from Glossogyne tenuifolia, induced apoptosis in A549 lung cancer cells
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