Effects of inoculum, delayed loading, and storage temperature of blood culture bottles on the detection of Burkholderia pseudomallei

Abstract Detection of Burkholderia pseudomallei in blood culture is critical to prompt appropriate treatment. Various factors may affect culture positivity. This study evaluated effects of various inocula (0.2–100 CFU/mL), delayed bottle loading periods (12–48 h), and storage temperatures (ambient,...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2008-12, Vol.62 (4), p.467-470
Main Author: Kiratisin, Pattarachai
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Blood culture
Burkholderia pseudomallei
Burkholderia pseudomallei - isolation & purification
Delayed entry
Fundamental and applied biological sciences. Psychology
Humans
Infectious Disease
Infectious diseases
Inoculum
Internal Medicine
Medical sciences
Melioidosis - blood
Melioidosis - diagnosis
Melioidosis - microbiology
Microbiology
Miscellaneous
Specimen Handling - methods
Temperature
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Summary:Abstract Detection of Burkholderia pseudomallei in blood culture is critical to prompt appropriate treatment. Various factors may affect culture positivity. This study evaluated effects of various inocula (0.2–100 CFU/mL), delayed bottle loading periods (12–48 h), and storage temperatures (ambient, 4 and 35 °C) for the detection of B. pseudomallei by the BacT/ALERT system. The results suggested that bottles may be preincubated at 35 °C for up to 12 h, or held at ambient temperature for up to 24 h, or refrigerated at 4 °C for up to 48 h.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2008.08.013