Hepatic amino- to urea-N clearance and forearm amino-N exchange during hypoglycemic and euglycemic hyperinsulinemia in normal man

Background/Aims: Hypoglycemia has well-described effects on glucose metabolism, whereas the possible effects on hepatic amino nitrogen conversion in relation to muscle amino nitrogen flux are more uncertain. Methods: We studied six healthy young male subjects three times, i.e. for 6 h in the basal s...

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Bibliographic Details
Published in:Journal of hepatology 1999-05, Vol.30 (5), p.819-825
Main Authors: Grøfte, Thorbjørn, Wolthers, Troels, Jørgensen, Jens Otto Lunde, Poulsen, Per Løgstrup, Vilstrup, Hendrik, Møller, Niels
Format: Article
Language:English
Subjects:
Adult
Alanine - blood
Amino Acids - blood
Amino-N metabolism
Biological and medical sciences
Blood Glucose - metabolism
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Forearm - blood supply
Glucagon - blood
Glucose Clamp Technique
Humans
Hyperinsulinism - physiopathology
Hypoglycemia
Hypoglycemia - physiopathology
Hypoglycemic Agents - administration & dosage
Hypoglycemic Agents - pharmacology
Infusions, Intravenous
Insulin - administration & dosage
Insulin - pharmacology
Insulin, Regular, Pork
Liver - metabolism
Male
Medical sciences
Nitrogen - metabolism
Tumors. Hypoglycemia
Urea - metabolism
Urea synthesis
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Summary:Background/Aims: Hypoglycemia has well-described effects on glucose metabolism, whereas the possible effects on hepatic amino nitrogen conversion in relation to muscle amino nitrogen flux are more uncertain. Methods: We studied six healthy young male subjects three times, i.e. for 6 h in the basal state, during a 6-h euglycemic hyperinsulinemic (1.5 mU/kg/min) clamp and during a 6-h hypoglycemic (plasma glucose below 2.8 mmol/l) clamp. Alanine (2 mmol/kg body weight/h) was infused for 3 h to describe the relationship between blood amino nitrogen concentrations and hepatic ureagenesis estimated from urea urine excretion and accumulation in body water. The slope of this relationship is denoted functional hepatic nitrogen clearance (FHNC) and quantifies substrate-independent alterations in hepatic amino nitrogen degradation. In parallel, amino nitrogen balances across muscles were estimated by the forearm flux method. Results: Euglycemia decreased circulating glucagon values (100±25 ng/l vs. 160±30 ng/l), whereas hypoglycemia doubled glucagon (350±45 ng/l, p
ISSN:0168-8278
1600-0641
DOI:10.1016/S0168-8278(99)80134-2