New Axonemal Dynein Heavy Chains from Tetrahymena thermophila

ABSTRACT Two dyneins can be extracted from Tetrahymena ciliary axonemes. The 22S dynein contains three heavy chains (HC), sediments at 22S in a sucrose gradient, and makes up the outer arms. The 14S dynein contains two to six HCs, sediments at 14S, and is thought to contribute to formation of the in...

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Published in:The Journal of eukaryotic microbiology 1999-03, Vol.46 (2), p.147-154
Main Authors: MOBBERLEY, PAULA S., SULLIVAN, JEANELL L., ANGUS, STEVEN P., KONG, XUEJUN, PENNOCK, DAVID G.
Format: Article
Language:English
Subjects:
Animals
Axoneme
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Blotting, Western
cell motility
Centrifugation, Density Gradient
cilia
Cilia - chemistry
Dyneins - chemistry
Dyneins - isolation & purification
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Protozoa
Tetrahymena thermophila
Tetrahymena thermophila - chemistry
Tetrahymena thermophila - growth & development
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Summary:ABSTRACT Two dyneins can be extracted from Tetrahymena ciliary axonemes. The 22S dynein contains three heavy chains (HC), sediments at 22S in a sucrose gradient, and makes up the outer arms. The 14S dynein contains two to six HCs, sediments at 14S, and is thought to contribute to formation of the inner arms. We have identified two large proteins that are extracted from Tetrahymena axonemes with high salt and that sediment together at approximately 18S. The two large proteins cleave when subjected to UV light in the presence of ATP and vanadate, suggesting both proteins are dynein HC. Antibodies against one of the 18S HCs do not recognize 22S dynein HCs. Antibodies to 22S dynein HC do not bind appreciably to 18S dynein photocleavage fragments. Taken together, these results indicate that the large proteins that sediment at 18S are axonemal dynein heavy chains.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.1999.tb04598.x