Identification of putative chromosomal origins of replication in Archaea

The mechanisms of DNA replication initiation are quite different in bacteria and eukarya. In bacteria, initiation occurs at a single locus, oriC, and is triggered by a single protein, DnaA, whereas, in eukarya, initiation takes place at multiple replication origins that are permanently occupied by o...

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Bibliographic Details
Published in:Molecular microbiology 1999-05, Vol.32 (4), p.883-886
Main Authors: Lopez, Philippe, Philippe, Hervé, Myllykallio, Hannu, Forterre, Patrick
Format: Article
Language:English
Subjects:
Archaea
Archaea - genetics
Chromosomes - genetics
Codon
DNA Replication - genetics
DNA-Binding Proteins
Methanobacterium - genetics
Origin Recognition Complex
Physical Chromosome Mapping
Pyrococcus - genetics
Repetitive Sequences, Nucleic Acid
Sequence Alignment
Viral Proteins
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Summary:The mechanisms of DNA replication initiation are quite different in bacteria and eukarya. In bacteria, initiation occurs at a single locus, oriC, and is triggered by a single protein, DnaA, whereas, in eukarya, initiation takes place at multiple replication origins that are permanently occupied by origin of replication complexes (ORC) made up of five or six protein subunits. These ORCs are made competent for initiation by the loading of minichromosome maintenance proteins (MCM), an association that is triggered by the protein Cdc6. Until recently, nothing was known about the initiation of DNA replication in the third domain of life, the Archaea, not even whether they have single or multiple replication origins. This situation is changing with the advent of archaeal genomics. In particular, Cdc6/Orc1 and MCM homologues have been detected in completely sequenced archaeal genomes.
ISSN:0950-382X
1365-2958
DOI:10.1046/j.1365-2958.1999.01370.x