Cofilin mediates tight-junction opening by redistributing actin and tight-junction proteins

We previously found that capsaicin induces tight-junction (TJ) opening accompanied with cofilin dephosphorylation/activation in intestinal Caco-2 cells. Here, we examined the role of cofilin in TJ regulation, and analyzed the structural events that lead to TJ opening. We transfected Caco-2 cells wit...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-12, Vol.377 (3), p.921-925
Main Authors: Nagumo, Yoko, Han, Junkyu, Bellila, Amor, Isoda, Hiroko, Tanaka, Toshiyuki
Format: Article
Language:English
Subjects:
Actin alteration
Actins - metabolism
Caco-2 Cells
Capsaicin
Capsaicin - pharmacology
Cell Membrane Permeability - drug effects
Claudin-1
Cofilin
Cofilin 1 - genetics
Cofilin 1 - metabolism
Cytoskeleton - drug effects
Cytoskeleton - metabolism
Humans
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestinal Mucosa - ultrastructure
Membrane Proteins - metabolism
Myosins - metabolism
Occludin
Phosphoproteins - metabolism
Tight Junctions - drug effects
Tight Junctions - metabolism
Tight-junction
Zonula Occludens-1 Protein
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Summary:We previously found that capsaicin induces tight-junction (TJ) opening accompanied with cofilin dephosphorylation/activation in intestinal Caco-2 cells. Here, we examined the role of cofilin in TJ regulation, and analyzed the structural events that lead to TJ opening. We transfected Caco-2 cells with wild-type cofilin [cofilin(wt)] or its constitutively active mutant cofilin(S3A). We found that the decreases in transepithelial electrical resistance (TER) was slower in cofilin(wt) transfectants and faster in cofilin(S3A) mutants than in vector controls. Moreover, cofilin dephosphorylation corresponded to the rate of TER decrease. Capsaicin treatment changed the localization of TJ proteins and altered the F-actin structure, but in a manner different from those depend on myosin light chain kinase (MLCK). These results strongly support the importance of cofilin in TJ opening, suggesting cofilin as a target for TJ permeability regulation in epithelial cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.10.071