Analysis of the Requirement for a pUB110 mob Region during Tn916-Dependent Mobilization

Tn916-dependent mobilization of nonconjugative plasmids pUB110 and its derivative pUB110Δm was compared. Deleting a 787-bp fragment from the pUB110 mob region created plasmid pUB110Δm. Deletion of the mob region of pUB110 rendered the plasmid nontransferable by the conjugative plasmids of Bacillus t...

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Bibliographic Details
Published in:Plasmid 1999-05, Vol.41 (3), p.179-186
Main Authors: Showsh, Sasha A., Andrews, Robert E.
Format: Article
Language:English
Subjects:
Bacillus subtilis - genetics
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Base Sequence
Conjugation, Genetic
DNA Transposable Elements
DNA, Bacterial - genetics
Escherichia coli - genetics
Plasmids - genetics
Sequence Deletion
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Summary:Tn916-dependent mobilization of nonconjugative plasmids pUB110 and its derivative pUB110Δm was compared. Deleting a 787-bp fragment from the pUB110 mob region created plasmid pUB110Δm. Deletion of the mob region of pUB110 rendered the plasmid nontransferable by the conjugative plasmids of Bacillus thuringiensis subsp. israelensis. During matings between Bacillus subtilis (Tn916) and B. thuringiensis subsp. israelensis, however, Tn916-dependent mobilization of plasmids pUB110 and pUB110Δm was observed at a frequency of approximately 2 × 10−6 transconjugants per donor. The results show that Tn916-mediated conjugal transfer of plasmids is a mob-independent event. Jaworski and Clewell (J. Bacteriol 177; 6644–6651) recently demonstrated the presence of an IncP-like nicking site in the oriT of Tn916. These data suggest that a IncP-like nickling site is essential for Tn916-mediated plasmid transfer.
ISSN:0147-619X
1095-9890
DOI:10.1006/plas.1999.1398