A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization

• Published in: Experimental cell research 2008-12, Vol.314 (20), p.3692-3700
• Main Authors: Kim, Kyeongmi, Lee, Seongju, Chang, Jaerak, Rhee, Kunsoo
• Format: Article
• Language: English
• Subjects: Autoantigens - metabolism; C-NAP1; Carrier Proteins - genetics; Carrier Proteins - metabolism; Carrier Proteins - physiology; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Cycle Proteins - physiology; Cell Division - physiology; Cells, Cultured; Cellular biology; Centrioles - metabolism; Centrosome; Centrosome split; CEP135; HeLa Cells; Humans; Models, Biological; Mutant Proteins - genetics; Mutant Proteins - metabolism; Mutation; Protein Binding; Protein Transport; Proteins; Proteomics; Transfection
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2008.09.016

A proteomic study predicted that about one hundred kinds of proteins constitute a basic structure of the centrosome. Most of the core centrosomal proteins contain extensive coiled-coil domains, suggesting that the protein–protein interaction is a critical force for the core centrosome configuration. In the present study, we investigated a novel interaction between CEP135 and C-NAP1, two core centriolar proteins. Depletion of CEP135 caused a premature centrosome splitting. Reduction of the centrosomal C-NAP1 level was accompanied in a specific manner. Ectopic expression of the CEP135 mutant proteins also caused centrosome splitting in association with the reduction of the centrosomal C-NAP1 levels. Based on these results, we propose that CEP135 acts as a platform protein for C-NAP1 at the centriole.