Chromosomal localization of a novel repetitive sequence in the Chenopodium quinoa genome

In this study, a novel repetitive sequence pTaq10 was isolated from the Taq I digest of the genomic DNA of the pseudocereal Chenopodium quinoa. Sequence analysis indicated that this 286-bp monomer is not homologous to any known retroelement sequence. FISH and Southern blot analysis showed that this...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied genetics 2008, Vol.49 (4), p.313-320
Main Authors: Kolano, Bozena, Plucienniczak, Andrzej, Kwasniewski, Miroslaw, Maluszynska, Jolanta
Format: Article
Language:English
Subjects:
Base Sequence
Chenopodium quinoa
Chenopodium quinoa - genetics
Chromosome Mapping
Chromosomes, Plant
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a novel repetitive sequence pTaq10 was isolated from the Taq I digest of the genomic DNA of the pseudocereal Chenopodium quinoa. Sequence analysis indicated that this 286-bp monomer is not homologous to any known retroelement sequence. FISH and Southern blot analysis showed that this sequence is characterized by an interspersed genomic organization. After FISH, hybridization signals were observed as small dots spread throughout all of the chromosomes. pTaq hybridization signals were excluded from 45S rRNA gene loci, but they partly overlapped with 5S rDNA loci. pTaq10 is not a species-specific sequence, as it was also detected in C. berlandieri.
ISSN:1234-1983
DOI:10.1007/BF03195629