An Electrochemiluminescence Flow-Through Cell and Its Applications to Sensitive Immunoassay Using N-(Aminobutyl)-N-ethylisoluminol

An electrochemiluminescence (ECL) flow-through cell with a carbon fiber electrode for ECL detection was developed, and a flow injection analysis system containing the cell was established. N-(aminobutyl)-N-ethylisoluminol (ABEI), an ECL reagent, was determined by this system. A straight-line calibra...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1999-06, Vol.71 (11), p.2237-2240
Main Authors: Arai, Kensuke, Takahashi, Kouji, Kusu, Fumiyo
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Fundamental and applied biological sciences. Psychology
Humans
Immunoassay - methods
Immunoglobulin G - analysis
Luminescent Measurements
Luminol - analogs & derivatives
Luminol - chemistry
Pharmacology
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Summary:An electrochemiluminescence (ECL) flow-through cell with a carbon fiber electrode for ECL detection was developed, and a flow injection analysis system containing the cell was established. N-(aminobutyl)-N-ethylisoluminol (ABEI), an ECL reagent, was determined by this system. A straight-line calibration curve for ABEI (r = 0.999) was obtained from 6 fmol to 25 pmol. The detection limit of ABEI was 6 fmol (S/N = 2) and the relative standard deviation was 1.7% at 1.5 pmol (n = 10). The system was used for immunoassay of human immunogloblin G (hIgG) with ABEI-labeled anti-hIgG. A calibration curve of hIgG was obtained from 80 pg/mL to 1.3 ng/mL. The detection limit of hIgG was 80 pg/mL (S/N = 2), and the relative standard deviation was 1.8% at 0.5 ng/mL (n = 10). Sensitivity and accuracy for sera samples were found to considerably exceed those of the conventional methods, such as single-radial immunodiffusion and nepherometric immunoassay. The present system should prove useful for immunoassay.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9810361