Identification of peptides presented by HLA class I molecules on cervical cancer cells with HPV-18 infection

• Published in: Immunology letters 1999-04, Vol.67 (3), p.167-177
• Main Authors: Monroy-Garcı́a, Alberto, Ortı́z-Navarrete, Vianney Francisco, Mora-Garcı́a, Marı́a de Lourdes, Flores-Borja, Fabián, Diaz-Quiñonez, Alberto, Isibasi-Araujo, Armando, Trejo-Becerril, Catalina, Chacón-Salinas, Rommel, Hernández-Montes, Jorge, Granados-Arreola, Julio, de Leo, Claudia, Weiss-Steider, Benny
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antigens, Viral - chemistry; Antigens, Viral - immunology; Cervical cancer cells; Cytotoxicity, Immunologic; Epitopes - immunology; Female; Histocompatibility Antigens Class I - chemistry; Histocompatibility Antigens Class I - immunology; HLA class I bound peptides; Human papillomavirus; Humans; Lymphocyte Activation; Mass Spectrometry; Molecular Sequence Data; Papillomaviridae - immunology; Papillomavirus Infections - immunology; Papillomavirus Infections - virology; Peptides - chemistry; Peptides - immunology; Peptides - isolation & purification; T-Lymphocytes - immunology; Tumor Cells, Cultured; Tumor Virus Infections - immunology; Tumor Virus Infections - virology; Uterine Cervical Neoplasms - immunology; Uterine Cervical Neoplasms - virology; viral antigens; Viral Proteins - chemistry; Viral Proteins - immunology; Viral Proteins - isolation & purification
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/S0165-2478(98)00188-6

In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.