iNOS -derived Nitric Oxide Modulates Infection-stimulated Bone Loss

Nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) plays an important role in host defense, as well as in inflammation-induced tissue lesions. Here we evaluated the role of NO in bone loss in bacterial infection-induced apical periodontitis by using iNOS-deficient mice (iNOS−/−)....

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Bibliographic Details
Published in:Journal of dental research 2008-12, Vol.87 (12), p.1155-1159
Main Authors: Fukada, S.Y., Silva, T.A., Saconato, I.F., Garlet, G.P., Ávila-Campos, M.J., Silva, J.S., Cunha, F.Q.
Format: Article
Language:English
Subjects:
Acid Phosphatase - analysis
Actinomycosis - enzymology
Alveolar Bone Loss - enzymology
Alveolar Bone Loss - pathology
Animals
Bacterial Infections - enzymology
Bacteroidaceae Infections - enzymology
Biomarkers - analysis
Cell Count
Cell Movement
Chemokine CXCL12 - analysis
Dental Pulp Exposure - microbiology
Dentistry
Isoenzymes - analysis
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - metabolism
Osteoclasts - pathology
Osteolysis - metabolism
Osteolysis - pathology
Osteoprotegerin - analysis
Periapical Periodontitis - enzymology
Periapical Periodontitis - pathology
RANK Ligand - analysis
Receptor Activator of Nuclear Factor-kappa B - analysis
Tartrate-Resistant Acid Phosphatase
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Summary:Nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) plays an important role in host defense, as well as in inflammation-induced tissue lesions. Here we evaluated the role of NO in bone loss in bacterial infection-induced apical periodontitis by using iNOS-deficient mice (iNOS−/−). The iNOS−/− mice developed greater inflammatory cell recruitment and osteolytic lesions than WT mice. Moreover, tartrate-resistant acid-phosphatase-positive (TRAP+) osteoclasts were significantly more numerous in iNOS−/− mice. Furthermore, the increased bone resorption in iNOS−/− mice also correlated with the increased expression of receptor activator NF-kappaB (RANK), stromal-cell-derived factor-1α (SDF-1α/CXCL12), and reduced expression of osteoprotegerin (OPG). These results show that NO deficiency was associated with an imbalance of bone-resorption-modulating factors, leading to severe infection-stimulated bone loss.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910808701207