Tools of the trade: diagnostics and research in domestic animal cytogenetics

This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase ch...

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Bibliographic Details
Published in:Journal of applied genetics 2008-01, Vol.49 (4), p.357-366
Main Authors: Iannuzzi, Leopoldo, Di Berardino, Dino
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Animals, Domestic - genetics
Biomedical and Life Sciences
Bovidae
Chromosome Banding
Cytogenetic Analysis
Cytogenetics - methods
Human Genetics
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
Review Article
Veterinary Medicine - methods
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Summary:This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.
ISSN:1234-1983
2190-3883
DOI:10.1007/BF03195634