Pulmonary diffusing capacity, serum angiotensin-converting enzyme activity and the angiotensin-converting enzyme gene in Japanese non-insulin-dependent diabetes mellitus patients

• Published in: Diabetes research and clinical practice 1999-03, Vol.43 (3), p.173-177
• Main Authors: Isotani, Haruhiko, Nakamura, Yoshio, Kameoka, Keiichi, Tanaka, Koji, Furukawa, Keizo, Kitaoka, Haruko, Ohsawa, Nakaaki
• Format: Article
• Language: English
• Subjects: ACE; ACE gene; Associated diseases and complications; Biological and medical sciences; Colorimetry; Diabetes Mellitus, Type 2 - physiopathology; Diabetes. Impaired glucose tolerance; Diabetic Retinopathy - physiopathology; DLCO; DNA - chemistry; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Female; Genotype; Humans; Japan; Male; Medical sciences; Microangiopathy; Middle Aged; NIDDM; Peptidyl-Dipeptidase A - blood; Peptidyl-Dipeptidase A - genetics; Polymerase Chain Reaction; Pulmonary Diffusing Capacity; Pulmonary function disorder
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/S0168-8227(99)00006-6

We investigated the independent change in pulmonary diffusing capacity (DLCO) as one manifestation of pulmonary microangiopathy and to analyze the correlation between DLCO and serum ACE. We also examined the association between DLCO and the ACE genes. We examined pulmonary functions, especially %DLCO/VA (DLCO corrected by alveolar volume, percent predicted) in 54 NIDDM patients and 34 age-matched normal control subjects. Subjects were subdivided according to the degree of retinopathy. Serum ACE level was assayed by a colorimetric method in 54 patients and an insertion/deletion polymorphism in the ACE gene was amplified using the polymerase chain reaction in 52 of the 54 patients. There was a significant reduction of %DLCO/VA (percent predicted P