Migration Inhibitory Factor Related Protein-8 (MRP-8) is an Autocrine Chemotactic Factor for Periodontal Ligament Cells

• Published in: Journal of dental research 1999-06, Vol.78 (6), p.1251-1255
• Main Authors: Nishimura, F., Terranova, V.P., Sawa, T., Murayama, Y.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antigens, Differentiation - analysis; Antigens, Differentiation - genetics; Antigens, Differentiation - pharmacology; Autocrine Communication; Calcium-Binding Proteins - analysis; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - pharmacology; Calgranulin A; Cell Movement; Cells, Cultured; Chemotactic Factors - analysis; Chemotactic Factors - genetics; Chemotactic Factors - pharmacology; Dentistry; Epidermal Growth Factor - pharmacology; Humans; Insulin-Like Growth Factor I - pharmacology; Insulin-Like Growth Factor II - pharmacology; Macrophage Migration-Inhibitory Factors - analysis; Macrophage Migration-Inhibitory Factors - genetics; Macrophage Migration-Inhibitory Factors - pharmacology; Molecular Sequence Data; Monocytes - metabolism; Neutrophils - metabolism; Periodontal Ligament - cytology; Periodontal Ligament - metabolism; Platelet-Derived Growth Factor - pharmacology; RNA, Messenger - analysis; Sequence Homology, Amino Acid
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/00220345990780060901

Previous studies have suggested that human periodontal ligament (PDL) cells secrete a chemotactic factor which stimulates motility in an autocrine manner. Here we report the partial amino acid sequence of a purified factor which shows 100% homology with human migration inhibitory factor related protein-8 (MRP-8). In addition, reverse-transcription polymerase chain-reaction (RT-PCR) analysis revealed that mRNA encoding MRP-8 was expressed in cultured human PDL cells. To confirm that MRP-8 is chemotactic for PDL cells, we synthesized 25 mer peptides overlapped by 5 amino acids covering the entire MRP-8 protein and tested them for their chemotactic activities. The data indicated that amino acid residues 21-45 showed chemotactic activity for cultured human PDL cells. The maximum chemotactic response was observed at the concentration of 10-15 mol/mL for human PDL cells. The chemotactic activity was estimated to be approximately 1000-fold higher than that of platelet-derived growth factor (PDGF), insulin-like growth factors-I and -II (IGF-I, -II), and epidermal growth factor (EGF) when compared on a molar basis. Since MRP-8 is reported to be produced mainly by neutrophils and monocytes, the result of the current study may suggest another important role of MRP-8 in human PDL cells.