Characterization of tyrosine sulfate residues in antihemophilic recombinant factor VIII by liquid chromatography electrospray ionization tandem mass spectrometry and amino acid analysis

Recombinant Factor VIII (rFVIII) is involved in the cascade of biochemical reactions leading to blood coagulation and is used for the treatment of haemophilia A. Plasma‐derived FVIII (pdFVIII) has been reported to be post‐translationally modified by sulfation of tyrosine residues at positions 346, 1...

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Published in:Rapid communications in mass spectrometry 1999-01, Vol.13 (11), p.1016-1023
Main Authors: Severs, Joanne C., Carnine, Mechelle, Eguizabal, Hugo, Mock, Kuldip K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - analysis
Chromatography, High Pressure Liquid
Factor VIII - analysis
Hydrolysis
Mass Spectrometry
Molecular Sequence Data
Peptide Fragments - analysis
Peptide Mapping
Recombinant Proteins - analysis
Spectrophotometry, Ultraviolet
Thrombin - chemistry
Tyrosine - analogs & derivatives
Tyrosine - analysis
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container_issue 11
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container_title Rapid communications in mass spectrometry
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creator Severs, Joanne C.
Carnine, Mechelle
Eguizabal, Hugo
Mock, Kuldip K.
description Recombinant Factor VIII (rFVIII) is involved in the cascade of biochemical reactions leading to blood coagulation and is used for the treatment of haemophilia A. Plasma‐derived FVIII (pdFVIII) has been reported to be post‐translationally modified by sulfation of tyrosine residues at positions 346, 1664, 1680, 718, 719 and 723.1 This report describes the quantitation of tyrosine sulfate residues in BHK‐derived, human rFVIII by amino acid composition analysis and the identification of their positions in the polypeptide sequence using a combination of liquid chromatography and electrospray ionization mass spectrometry in the analysis of proteolytic digests of the protein. Copyright © 1999 John Wiley & Sons, Ltd.
doi_str_mv 10.1002/(SICI)1097-0231(19990615)13:11<1016::AID-RCM599>3.0.CO;2-5
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source Wiley-Blackwell Read & Publish Collection
subjects Amino Acid Sequence
Amino Acids - analysis
Chromatography, High Pressure Liquid
Factor VIII - analysis
Hydrolysis
Mass Spectrometry
Molecular Sequence Data
Peptide Fragments - analysis
Peptide Mapping
Recombinant Proteins - analysis
Spectrophotometry, Ultraviolet
Thrombin - chemistry
Tyrosine - analogs & derivatives
Tyrosine - analysis
title Characterization of tyrosine sulfate residues in antihemophilic recombinant factor VIII by liquid chromatography electrospray ionization tandem mass spectrometry and amino acid analysis
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