Ion-exclusion/cation-exchange chromatographic determination of common inorganic ions in human saliva by using an eluent containing zwitterionic surfactant

Ion-exclusion/cation-exchange chromatography with an eluent containing the bile salt-type zwitterionic surfactant CHAPS was performed in order to evaluate variations in anion (SO 4 2−, NO 3 −, and SCN −) and cation (Na +, K +, NH 4 +, Mg 2+, and Ca 2+) concentrations in human saliva. CHAPS prevents...

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Bibliographic Details
Published in:Journal of Chromatography A 2008-12, Vol.1213 (2), p.125-129
Main Authors: Mori, Masanobu, Iwata, Tomotaka, Satori, Tatsuya, Ohira, Shin-Ichi, Itabashi, Hideyuki, Tanaka, Kazuhiko
Format: Article
Language:English
Subjects:
Anions - analysis
Biological and medical sciences
Calcium - analysis
Cations - analysis
Cholic Acids - chemistry
Chromatography, Gel - methods
Chromatography, Ion Exchange - methods
Humans
Inorganic ions
Investigative techniques, diagnostic techniques (general aspects)
Ion-exclusion/cation-exchange chromatography
Magnesium - analysis
Medical sciences
Miscellaneous. Technology
Nitrates - analysis
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Potassium - analysis
Quaternary Ammonium Compounds - analysis
Saliva
Saliva - chemistry
Smoking
Sodium - analysis
Sulfates - analysis
Thiocyanates - analysis
Water analysis
Zwitterionic surfactant
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Summary:Ion-exclusion/cation-exchange chromatography with an eluent containing the bile salt-type zwitterionic surfactant CHAPS was performed in order to evaluate variations in anion (SO 4 2−, NO 3 −, and SCN −) and cation (Na +, K +, NH 4 +, Mg 2+, and Ca 2+) concentrations in human saliva. CHAPS prevents the adsorption of proteins to the stationary phase, i.e., weakly acidic cation-exchange resin, since it aggregates proteins without denaturing them. Addition of 1 mM CHAPS to the eluent comprising 6 mM tartaric acid and 7 mM 18-crown-6 yielded reproducible separations of anions and cations in protein-containing saliva. The resolutions of anions and cations were not significantly affected by the addition of CHAPS to the eluent. The concentrations of Na + and K + varied before and after meals; or that of SCN −, upon smoking. The relative standard deviations of peak areas ranged from 0.3 to 5.1% in 1 day ( n = 20) and from 1.4 to 5.8% over 6 days ( n = 6).
ISSN:0021-9673
DOI:10.1016/j.chroma.2008.08.001