E-cadherin core fucosylation regulates nuclear β-catenin accumulation in lung cancer cells

• Published in: Glycoconjugate journal 2008-12, Vol.25 (9), p.843-850
• Main Authors: Hu, Ping, Shi, Bizhi, Geng, Fei, Zhang, Chunyi, Wu, Wei, Wu, Xing Zhong
• Format: Article
• Language: English
• Subjects: beta Catenin - genetics; beta Catenin - metabolism; Biochemistry; Biomedical and Life Sciences; Cadherins - metabolism; Cell Line, Tumor; Cell Nucleus - metabolism; Core fucosyltransferase; E-cadherin; Fucose - metabolism; Fucosyltransferases - metabolism; Gene Expression Regulation, Neoplastic; Humans; Life Sciences; Lung Neoplasms - enzymology; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Pathology; Phosphotyrosine - metabolism; Protein Binding; Protein Processing, Post-Translational; TCF Transcription Factors - genetics; Transcription, Genetic; Transfection; β-catenin
• ISSN: 0282-0080; 1573-4986
• DOI: 10.1007/s10719-008-9144-6

E-cadherin expressed highly in 95C and lowly in 95D lung cancer cells which were from the same patient, but core-fucosylated E-cadherin highly expressed in 95D cells. Therefore, Fut8 and Fut8-RNAi constructs were transfected into 95C and 95D cells, respectively. In Fut8-transfectants, reduction of nuclear β-catenin was noted when E-cadherin was core-fucosylated, while accumulation of nuclear β-catenin was observed in Fut8-RNAi transfectants. In E-cadherin-negative MDA-MB-231 cells either Fut8 or Fut8-RNAi transfection couldn't affect nuclear β-catenin. However, cotransfection of E-cadherin with Fut8 caused nuclear β-catenin reduction. Furthermore, enhanced binding of E-cadheirn with β-catenin as well as α-catenin were observed in Fut8-transfectants, and reduction of tyrosine 654 phosphorylation on β-catenin and its transcriptional activity were also noted at the same time. Overall, the current results suggested that core-fucosylated E-cadherin regulated nuclear β-catenin accumulation in lung cancer cells.