Isolation and Mapping of Novel Candidate Genes for Retinal Disorders Using Suppression Subtractive Hybridization

• Published in: Genomics (San Diego, Calif.) Calif.), 1999-06, Vol.58 (3), p.240-249
• Main Authors: den Hollander, Anneke I., van Driel, Marc A., de Kok, Yvette J.M., van de Pol, Dorien J.R., Hoyng, Carel B., Brunner, Han G., Deutman, August F., Cremers, Frans P.M.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Choroid - metabolism; Chromosome Mapping; Cricetinae; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Gene Library; Genes - genetics; Genes. Genome; Humans; Hybrid Cells; Medical sciences; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleic Acid Hybridization - methods; Ophthalmology; Pigment Epithelium of Eye - metabolism; retina; Retinal Diseases - genetics; retinal pigment epithelium; Retinopathies; RP12; RP17; Sensitivity and Specificity; Sequence Analysis, DNA; suppression subtractive hybridization
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1999.5823

We have constructed human cDNA libraries enriched for retina- and retinal pigment epithelium (RPE)/choroid-specific cDNAs through suppression subtractive hybridization. The sequence of 314 cDNAs from the retina enriched library and 126 cDNAs from the RPE/choroid enriched library was analyzed. Based on the absence of a database match, 25% of the retina cDNA clones and 16% of the RPE/choroid cDNA clones are novel cDNAs. The expression profiles of 86 retina and 21 RPE/choroid cDNAs were determined by a semiquantitative reverse transcription polymerase chain reaction technique. Thirty-three cDNAs were expressed exclusively or most prominently in retina or RPE/choroid. These cDNAs were mapped in the human genome by radiation hybrid mapping. Eleven cDNAs colocalized with loci involved in retinal disorders. One cDNA mapped in a 1.5-megabase critical region for autosomal recessive retinitis pigmentosa (RP12). Another cDNA was assigned to the 7.7-cM RP17 linkage interval. Seven cDNAs colocalized with four loci involved in Bardet–Biedl syndrome.