Comparison of C1q-receptors on rat microglia and peritoneal macrophages

A comparison of the expression and ligand specificity of the C1q (first complement component) receptor on rat microglia and peritoneal macrophages was made. This revealed that radiolabelled C1q was competed from the peritoneal macrophages with intact C1q, and additively displaced by calf-skin collag...

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Bibliographic Details
Published in:Journal of neuroimmunology 1999-02, Vol.94 (1), p.74-81
Main Authors: Wing, M.G, Seilly, D.J, Nicholas, R.S, Rahman, S, Zajicek, J, Lachmann, P.J, Compston, D.A.S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding, Competitive - immunology
C1q peptide
C1q receptor
Carrier Proteins
Collagen - pharmacology
Collagenases - genetics
Collagenases - pharmacology
Complement C1q - metabolism
Complement C1q - pharmacology
Electrophoresis, Polyacrylamide Gel
Humans
Hyaluronan Receptors
Macrophage
Macrophages, Peritoneal - chemistry
Macrophages, Peritoneal - immunology
Membrane Glycoproteins
Microglia
Microglia - chemistry
Microglia - immunology
Mitochondrial Proteins
Molecular Sequence Data
Rats
Rats, Wistar
Receptors, Complement - analysis
Receptors, Complement - metabolism
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Summary:A comparison of the expression and ligand specificity of the C1q (first complement component) receptor on rat microglia and peritoneal macrophages was made. This revealed that radiolabelled C1q was competed from the peritoneal macrophages with intact C1q, and additively displaced by calf-skin collagen and purified C1q globular heads, suggesting the presence of at least two receptors. This was in contrast to microglia, where radiolabelled C1q was displaced with intact C1q and to a modest degree with collagen, but not with globular heads. Taken together, this implies that under these conditions, peritoneal macrophages and microglia both express a C1q receptor which binds to the collagen-like region, and that peritoneal macrophages additionally express a molecule which binds to the globular head of C1q. Analysis of the ligand bound by these cells reflected the differences observed in the competitive binding experiments, with the novel identification of naturally-occurring peptides from the globular head of C1q bound to the peritoneal macrophages, but not the microglia.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(98)00227-6