The effect of Helicobacter pylori on neutrophil chemotaxis is independent of cagA

• Published in: FEMS immunology and medical microbiology 1999-06, Vol.24 (2), p.209-213
• Main Authors: Akyön, Yakut, Hascelik, Gulsen
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Antigens, Bacterial; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; cagA; cagA gene; Chemotaxis, Leukocyte - immunology; Child; DNA, Bacterial - analysis; Duodenal ulcer; Duodenal Ulcer - immunology; Duodenal Ulcer - microbiology; Female; Fundamental and applied biological sciences. Psychology; Gastritis; Gastritis - immunology; Gastritis - microbiology; Helicobacter Infections - immunology; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - immunology; Helicobacter pylori - isolation & purification; Human bacterial diseases; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Neutrophil chemotaxis; Neutrophils - immunology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1016/S0928-8244(99)00028-0

Sixteen Helicobacter pylori strains were studied in order to determine their neutrophil chemotactic activity and the association with the presence cagA gene. Neutrophil chemotactic activity was detected by a modified Boyden chamber method and the results were expressed in terms of chemotactic index (CI). The presence of cagA was determined by PCR. Of the 16 strains, eight were cagA + and eight were cagA −. All of the isolated strains showed chemotactic activity. The mean value of CI of the patient group was significantly higher than the negative control ( P