Ultrastructural localization of hippocampal TNF-α immunoreactive cells in rats following transient global ischemia

Using a polyclonal antibody against rat TNF-α, we have identified specific intracellular immunoreactive sites in hippocampal pyramidal cells, astroglia, and in microglia within 72 h after a period of ischemia. Electron opaque immunoreactive products in pyramidal cells were found mainly in somata and...

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Bibliographic Details
Published in:Brain research 1999-06, Vol.833 (1), p.121-124
Main Authors: Tseng, Michael T., Chang, Chih C.
Format: Article
Language:English
Subjects:
Animals
Astrocytes - metabolism
Biological and medical sciences
Brain Ischemia - metabolism
Brain Ischemia - pathology
Glia
Hippocampus
Hippocampus - metabolism
Hippocampus - pathology
Hippocampus - ultrastructure
Immunoelectron Microscopy
Male
Medical sciences
Microglia - metabolism
Microscopy, Immunoelectron
Neurology
Pyramidal Cells - metabolism
Pyramidal Neuron
Rats
Rats, Long-Evans
Time Factors
Tissue Distribution - physiology
TNF-α
Tumor Necrosis Factor-alpha - metabolism
Vascular diseases and vascular malformations of the nervous system
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Summary:Using a polyclonal antibody against rat TNF-α, we have identified specific intracellular immunoreactive sites in hippocampal pyramidal cells, astroglia, and in microglia within 72 h after a period of ischemia. Electron opaque immunoreactive products in pyramidal cells were found mainly in somata and dendrites. Astrocytes and microglia were nearly devoid of such complexes. These findings demonstrate the presence of TNF-α in hippocampal neurons and its enhancement by ischemic stress.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(99)01490-0