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Effect of polydeoxyribonucleotides on human fibroblasts in primary culture

The effects of a mixture of oligo‐ and polydeoxyribonucleotides (PDRN) on the growth and protein secretion of cultured human skin fibroblasts were investigated. Both intact and DNAase‐digested PDRN stimulated cell proliferation to a similar extent. When cultured fibroblasts were incubated with radio...

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Bibliographic Details
Published in:Cell biochemistry and function 1999-06, Vol.17 (2), p.107-114
Main Authors: Sini, P., Denti, A., Cattarini, G., Daglio, M., Tira, M.E., Balduini, C.
Format: Article
Language:English
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Summary:The effects of a mixture of oligo‐ and polydeoxyribonucleotides (PDRN) on the growth and protein secretion of cultured human skin fibroblasts were investigated. Both intact and DNAase‐digested PDRN stimulated cell proliferation to a similar extent. When cultured fibroblasts were incubated with radioactive amino acids in the presence of intact or digested PDRN the incorporation of the tracer into secreted proteins increased significantly. This stimulation appears to be specific for certain protein components, including fibronectin. These results are interpreted assuming that PDRN and the nucleotides and nucleosides resulting from its degradation, can act as signal transducers or, alternatively, can be internalized and utilized to provide purine and pyrimidine rings for the salvage pathways. Copyright © 1999 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/(SICI)1099-0844(199906)17:2<107::AID-CBF815>3.0.CO;2-#